Pleiotrophin inhibits hippocampal long-term potentiation: A role of pleiotrophin in learning and memory
Pleiotrophin inhibits hippocampal long-term potentiation: A role of pleiotrophin in learning and memory
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DOI：
10.1080/08977190902906859
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发表时间：
2009-01-01
期刊：
影响因子：
1.8
通讯作者：
Herradon, Gonzalo
中科院分区：
文献类型：
--
作者：
del Olmo, Nuria;Gramage, Esther;Herradon, Gonzalo
多效素（PTN）是一种生长因子，已被证明与海马突触可塑性和学习有关。为了进一步了解 PTN 在记忆过程中的参与，我们对来自大鼠海马切片的 PTN 刺激的 CA1 进行了体外电生理学研究，并结合 PTN 缺陷 (PTN-/-) 小鼠的行为测试。我们发现 PTN 抑制由高频刺激 (HFS) 引起的海马长时程增强 (LTP)，包括 3 个 100Hz 的序列，间隔 20 秒。为了测试 PTN 可能参与行为记忆过程的可能性，我们使用 Y 迷宫测试测试了 PTN-/-小鼠的学习行为。当在 Y 迷宫测试中使用 30 分钟间隔间期 (ITI) 时，我们没有观察到 PTN-/- 和野生型 (WT) 小鼠的识别记忆存在显着差异。然而，WT 小鼠使用 60 分钟-ITI 显示识别记忆被破坏，而 PTN-/- 小鼠则保持了识别记忆。数据表明 PTN 在体外抑制海马 LTP，并可能在体内记忆过程中发挥作用。
Pleiotrophin (PTN) is a growth factor that has been shown to be involved in hippocampal synaptic plasticity and learning. To further understand the involvement of PTN in memory processes, we performed in vitro electrophysiological studies in PTN-stimulated CA1 from rat hippocampal slices combined with the behavioural testing of PTN deficient (PTN-/-) mice. We found that PTN inhibited hippocampal long-term potentiation (LTP) induced by high-frequency stimulation (HFS) consisted in three trains of 100Hz separated by 20s. To test the possibility that PTN might be involved in behavioural memory processes, we tested the learning behaviour of PTN-/-mice using the Y-maze test. We did not observe significant differences in recognition memory between PTN-/-and Wild Type (WT) mice when a 30min-interval intertrial (ITI) was used in the Y-maze test. However, whereas WT mice showed disruption of recognition memory using a 60min-ITI, PTN-/-mice maintained the recognition memory. The data demonstrate that PTN inhibits hippocampal LTP in vitro and might play a role in memory processes in vivo.