Proteomic Analysis of the Mouse Ovary in Response to Two Gonadotropins, Follicle-Stimulating Hormone and Luteinizing Hormone
Proteomic Analysis of the Mouse Ovary in Response to Two Gonadotropins, Follicle-Stimulating Hormone and Luteinizing Hormone
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DOI：
10.1262/jrd.20217
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发表时间：
2009-06-01
影响因子：
1.8
通讯作者：
Matsumoto, Kazuya
中科院分区：
文献类型：
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作者：
Satoh, Manabu;Tokoro, Mikiko;Matsumoto, Kazuya
哺乳动物卵巢的功能和结构变化受垂体糖蛋白激素、卵泡刺激素(FSH)和黄体生成素(LH)的协调调节，导致卵泡发育、排卵和卵泡转化为黄体。为了研究小鼠卵巢周期这些过程中的蛋白质谱，我们应用了组合方法(蛋白质分离和可视化的二维凝胶电泳法[2-DE]和蛋白质鉴定的基质激光解吸/电离飞行时间质谱仪[MALDI-TOF/MS]分析)，对3周龄的未成熟小鼠进行了比较蛋白质组学分析。从孕马血清促性腺激素(PMSG)/人绒毛膜促性腺激素(HCG)诱导的未成熟小鼠的完整卵巢中提取蛋白质，在0(无PMSG)、PMSG后24和48h以及hCG后10和20h获得蛋白质谱。结果表明，在pH为3~11和15~200 kDa范围内的代表性凝胶中，共有1028个蛋白质斑点，其中253个蛋白质斑点(24.6%)存在差异表达(P
Functional and structural changes in the mammalian ovary are coordinately regulated by the pituitary glycoprotein hormones, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), leading to follicular development, ovulation and transformation of follicles into corpus lutea. To investigate protein profiles during these processes of the mouse ovarian cycle, we applied combined methods (two-dimensional gel electrophoresis [2-DE] for separation and visualization of proteins plus matrix laser desorption/ionization time-of-flight mass spectrometry [MALDI-TOF/MS] analysis for protein identification) for comparative proteomic analysis using immature mice at 3 weeks of age. Protein profiles were obtained from proteins extracted from intact ovaries that had been collected from pregnant mare serum gonadotropin (PMSG)/human chorionic gonadotropin (hCG)-primed immature mice at 0 (no PMSG), 24 and 48 h post PMSG, as well as at 10 and 20 h post hCG. The results showed that 1028 common protein spots were found in representative gels that had been separated in the 3 to 11 pH range and the 15-200 kDa range, 253 protein spots (24.6%) of which were differentially expressed (p