A small-molecule dye for NIR-II imaging
A small-molecule dye for NIR-II imaging
复制标题
用于 NIR-II 成像的小分子染料
DOI：
10.1038/nmat4476
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发表时间：
2016-02-01
期刊：
影响因子：
41.2
通讯作者：
Dai, Hongjie
中科院分区：
文献类型：
--
作者：
Antaris, Alexander L.;Chen, Hao;Dai, Hongjie
第二近红外窗口（NIR-II）中的生物系统荧光成像可以探测厘米深度的组织，并在毫米深度实现微米级分辨率。不幸的是，目前所有的NIR-II荧光团排泄缓慢，并在很大程度上保留在网状内皮系统，使临床翻译几乎不可能。在这里，我们报告了一个快速排泄的NIR-II荧光团（类似于90%通过肾脏排泄在24小时内）的基础上合成的970-Da的有机分子（CH 1055）。荧光团优于吲哚菁绿色（ICG）-临床批准的近红外-I染料-在解决小鼠淋巴管和肿瘤附近的前哨淋巴映射。在小鼠脑肿瘤中观察到高水平的PEG化-CH 1055染料摄取，表明在接近4 mm的深度处检测到该染料。当与抗EGFR亲和体缀合时，CH 1055染料还允许体内肿瘤的靶向分子成像。此外，上级肿瘤与背景信号比允许精确的图像引导肿瘤切除手术。
Fluorescent imaging of biological systems in the second near-infrared window (NIR-II) can probe tissue at centimetre depths and achieve micrometre-scale resolution at depths of millimetres. Unfortunately, all current NIR-II fluorophores are excreted slowly and are largely retained within the reticuloendothelial system, making clinical translation nearly impossible. Here, we report a rapidly excreted NIR-II fluorophore (similar to 90% excreted through the kidneys within 24 h) based on a synthetic 970-Da organic molecule (CH1055). The fluorophore outperformed indocyanine green (ICG)-a clinically approved NIR-I dye-in resolving mouse lymphatic vasculature and sentinel lymphatic mapping near a tumour. High levels of uptake of PEGylated-CH1055 dye were observed in brain tumours in mice, suggesting that the dye was detected at a depth of similar to 4 mm. The CH1055 dye also allowed targeted molecular imaging of tumours in vivo when conjugated with anti-EGFR Affibody. Moreover, a superior tumour-to-background signal ratio allowed precise image-guided tumour-removal surgery.