Fasciculation and elongation protein zeta-1 (FEZ1) participates in the polarization of hippocampal neuron by controlling the mitochondrial motility
Fasciculation and elongation protein zeta-1 (FEZ1) participates in the polarization of hippocampal neuron by controlling the mitochondrial motility
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DOI：
10.1016/j.bbrc.2006.11.142
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发表时间：
2007-02-02
影响因子：
3.1
通讯作者：
Kuroda, Shun'ichi
中科院分区：
文献类型：
--
作者：
Ikuta, Junko;Maturana, Andres;Kuroda, Shun'ichi
Fasciculation and elongation protein zeta-1（FEZ 1）是秀丽隐杆线虫（Caeochrotrtocytoma elegans）的一种同源蛋白，分子量为45 kDa，具有4个卷曲螺旋结构域，能有效促进大鼠嗜铬细胞瘤PC 12细胞的神经突起伸长。C.线虫和果蝇在遗传学上已被证明参与轴突导向。我们在这里显示，FEZI RNA干扰（RNAi）抑制轴突在大鼠胚胎海马神经元的形成。在RNAi处理的海马神经元的神经突中，顺行线粒体运动也被阻滞。此外，通过RNAi处理，线粒体的大小显著延长。线粒体从索马体向轴突或树突的转运是神经元分化的必要条件。因此，我们的研究结果强烈表明，FEZ 1参与建立神经元极性通过控制线粒体运动沿着轴突。(c)2006年爱思唯尔公司All rights reserved.
The fasciculation and elongation protein zeta-1 (FEZ 1), a mammalian orthologue of Caenorhabditis elegans UNC-76 protean, is a 45-kDa protein with four coiled-coiled domains and efficiently promotes the neurite elongation in the rat phaeochrotrtocytoma PC12 cells. UNC-76 proteins of C. elegans and Drosophila have been genetically demonstrated to be involved in the axonal guidance. We here show that FEZI RNA interference (RNAi) represses the formation of axon in rat embryo hippocampal neurons. An anterograde mitochondrial movement is also retarded in neurites of the RNAi-treated hippocampal neurons. Moreover, the size of mitochondria is considerably elongated by the RNAi treatment. The transport of mitochondria from soma to axon or dendrites is essential for the neuronal differentiation. Therefore, our results strongly suggest that FEZ1 participates in the establishment of neuronal polarity by controlling the mitochondrial motility along axon. (c) 2006 Elsevier Inc. All rights reserved.