The erythromycin biosynthetic gene cluster of Aeromicrobium erythreum
The erythromycin biosynthetic gene cluster of Aeromicrobium erythreum
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DOI：
10.1007/s10295-004-0154-5
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发表时间：
2004-08-01
影响因子：
3.4
通讯作者：
Weber, JM
中科院分区：
文献类型：
--
作者：
Brikun, IA;Reeves, AR;Weber, JM
克隆并鉴定了红霉素气微菌（Aeromicrobium erythreum）红霉素生物合成基因簇（ery）。55.4kb的基因簇包含25个ery基因。同源物被发现为每个基因在以前的特征ery基因簇从多孢菌的葡萄球菌。此外，还鉴定了4个新的预测基因。两个新预测的基因，编码磷酸泛酰巯基乙胺基转移酶（eryP）和II型硫酯酶（eryTII），是ery簇的内部基因。另外两个新的基因，编码胸苷5 '-二磷酸葡萄糖合成酶（eryDI）和MarR家族转录抑制因子（ery-ORF 25），被发现在ery簇的两端。敲除eryDI表明它是红霉素生物合成所必需的。这两个基因簇的基因顺序在一个由15个相邻基因组成的核心区域内是保守的，只有IS 1136在A.刺桐丛在核心区域之外，基因簇的两侧发生了基因重排。两个ery簇的侧翼区域在发现的基因类型上并不相似。
The erythromycin-biosynthetic (ery) gene cluster of Aeromicrobium erythreum was cloned and characterized. The 55.4-kb cluster contains 25 ery genes. Homologues were found for each gene in the previously characterized ery gene cluster from Saccharopolyspora erythraea. In addition, four new predicted ery genes were identified. Two of the new predicted genes, coding for a phosphopantetheinyl transferase (eryP) and a type II thioesterase (eryTII), were internal to the ery cluster. The other two new genes, coding for a thymidine 5'-diphosphate-glucose synthase (eryDI) and a MarR-family transcriptional repressor (ery-ORF25), were found at the two ends of the ery cluster. A knockout in eryDI showed it to be essential for erythromycin biosynthesis. The gene order of the two ery clusters was conserved within a core region of 15 contiguous genes, with the exception of IS1136 which was not found in the A. erythreum cluster. Beyond the core region, gene shuffling had occurred between the two sides of the cluster. The flanking regions of the two ery clusters were not alike in the type of genes found.