Purification, characterization, and cDNA cloning of a galactose-specific C-type lectin from Drosophila melanogaster
Purification, characterization, and cDNA cloning of a galactose-specific C-type lectin from Drosophila melanogaster
复制标题
DOI：
10.1074/jbc.271.33.20213
复制
发表时间：
1996-08-16
影响因子：
4.8
通讯作者：
Natori, S
中科院分区：
文献类型：
--
作者：
Haq, S;Kubo, T;Natori, S
我们从果蝇蛹提取物中纯化了一种凝集素。该凝集素在钙或镁存在下能凝集胰蛋白酶化和戊二醛固定的牛红细胞，其半抗原糖为半乳糖，完整的凝集素分子量为41kDa，由14和17kDa亚基组成，其中17kDa亚基是14kDa亚基的糖基化形式。该凝集素的cDNA的分析表明，14 kDa的亚基由163个氨基酸残基组成，并包含在各种C型凝集素中保守的所有残基。果蝇凝集素和麻蝇凝集素在防御和发育方面具有相似的性质和功能，但可能不是结构上的同源物。
We purified a lectin from a pupal extract of Drosophila melanogaster. This lectin agglutinated trypsinized and glutaraldehyde-fixed bovine red blood cells in the presence of calcium or magnesium, The hapten sugar of this lectin was galactose, The molecular mass of the intact lectin was determined to be 41 kDa, and it comprised 14- and 17-kDa subunits, The 17-kDa subunit was shown to be a glycosylated form of the 14-kDa subunit. Analysis of the cDNA for this lectin revealed that the 14-kDa subunit consists of 163 amino acid residues and contains all residues conserved in various C-type lectins. It was suggested that the Drosophila lectin and Sarcophaga lectin share some properties and function similarly in defense and development, but probably they are not structural homologues.