Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method
Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method
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DOI：
10.1088/0022-3727/32/21/316
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发表时间：
1999-11-07
影响因子：
3.4
通讯作者：
Hara, M
中科院分区：
文献类型：
--
作者：
Suehiro, J;Yatsunami, R;Hara, M
我们描述了一种新的技术，实现了定量估计的生物细胞浓度在水介质中。提出的介电电泳阻抗测量方法利用正介电电泳力捕获悬浮的生物颗粒到珍珠链形成的叉指型微电极阵列上。更高的细胞浓度导致珍珠链的更快发展，其在电极间隙内并联电连接以增加电极之间的电导和电容。通过监测电阻抗的时间变化，可以根据细胞收集过程的理论模型定量评估细胞群体。实验证明，在10（5）cm（-3）浓度下，大肠杆菌悬液可在10分钟内准确测定。
We describe a new technique that realizes the quantitative estimation of the biological cell concentration in an aqueous medium. The proposed dielectrophoretic impedance measurement method utilizes the positive dielectrophoretic force to capture suspended biological particles onto an interdigitated micro-electrode array in pearl-chain formation. Higher cell concentration results in faster development of the pearl chains, which are electrically connected in parallel within the electrode gap to increase the conductance and capacitance between the electrodes. By monitoring temporal variation of the electrical impedance, it is possible to quantitatively evaluate the cell population according to a theoretical model of the cell collection process. It has been demonstrated that a suspension liquid of Escherichia coli could be accurately assayed in about 10 min at 10(5) cm(-3) concentration.