HEPATITIS-B VIRUS-DNA CONTAINS A GLUCOCORTICOID-RESPONSIVE ELEMENT
HEPATITIS-B VIRUS-DNA CONTAINS A GLUCOCORTICOID-RESPONSIVE ELEMENT
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DOI：
10.1073/pnas.83.6.1627
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发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
SHAFRITZ, DA
中科院分区：
文献类型：
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作者：
TURKASPA, R;BURK, RD;SHAFRITZ, DA
最近的研究表明，乙肝病毒(乙肝病毒)含有转录增强因子。为了确定该增强子是否对糖皮质激素有反应，构建了一系列含有HBV增强子和可变长度上游序列的pA10CAT2衍生子。将其导入PLC/PRF/5、Hep 3B、Hep G2、HeLa和小鼠L细胞，检测氯霉素乙酰转移酶(CAT)的瞬时表达。人肝细胞癌细胞系PLC/PRF/5含有完整的HBVDNA序列，CAT活性最高。地塞米松增加了所有细胞系中CAT的表达，在10 nM时最大诱导率为40%，在1微米地塞米松时最大刺激(3-8倍)。只有当含有HBVDNA序列的构建体位于EcoRI位点MAP位置735的上游时，才能观察到地塞米松对CAT表达的增强。这表明糖皮质激素反应区域不同于先前定义的位于MAP位置1080-1234的乙肝病毒增强子序列。这些研究表明，HBVDNA中含有糖皮质激素反应元件，该元件可能在感染的哺乳动物细胞中介导了HBVDNA的表达。
It has recently been shown that hepatitis B virus (HBV) contains a transcriptional enhancer element. In order to determine whether this enhancer responds to glucocorticoids, a series of derivatives of plasmid pA10CAT2 was constructed containing the HBV enhancer and variable lengths of further upstream sequences. Transient expression of chloramphenicol acetyltransferase (CAT) was determined after introduction of these plasmids into PLC/PRF/5, Hep 3B, Hep G2, HeLa, and mouse L cells. Highest CAT activity was noted in the human hepatocellular carcinoma line PLC/PRF/5, which contains integrated HBV DNA sequences. Dexamethasone augmented CAT expression in all cell lines tested with 40% of maximal induction at 10 nM and maximum stimulation (3- to 8-fold) at 1 .mu.M dexamethasone. Dexamethasone augmentation of CAT expression was observed only when constructs contained HBV DNA sequences residing upstream to map position 735 from the EcoRI site. This indicates that the glucocorticoid-responsive region is distinct from the previously defined HBV enhancer sequence located at map position 1080-1234. These studies suggest that HBV DNA contains a glucocorticoid-responsive element, which may mediate expression of HBV genes in infected mammalian cells.