MAPPING PATTERNS OF C-FOS EXPRESSION IN THE CENTRAL-NERVOUS-SYSTEM AFTER SEIZURE
MAPPING PATTERNS OF C-FOS EXPRESSION IN THE CENTRAL-NERVOUS-SYSTEM AFTER SEIZURE
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DOI：
10.1126/science.3037702
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发表时间：
1987-07-10
期刊：
影响因子：
56.9
通讯作者：
CURRAN, T
中科院分区：
文献类型：
--
作者：
MORGAN, JI;COHEN, DR;CURRAN, T
一个戏剧性的和具体的诱导c-fos观察到在体内可识别的神经元群体后，管理的惊厥美曲唑。这种效应具有时间和剂量依赖性，可通过抗惊厥药物地西泮或戊巴比妥预先治疗消除。美曲唑给药后60分钟左右，c-fos mRNA达到最大值，180分钟后降至基础水平。在16小时后恢复到基础水平之前，观察到低于正常脑的进一步降低。虽然美曲唑在此期间仍然引起癫痫发作，但c-fos的再诱导在很大程度上是难治性的。在90分钟时，在齿状回、梨状和扣带回皮质的神经元核中观察到c-fos蛋白。随后，c-fos蛋白出现在整个皮层，海马和边缘系统。因此，癫痫发作活动导致特定神经元亚群中c-fos基因表达增加。
A dramatic and specific induction of c-fos was observed in identifiable neuronal populations in vivo after administration of the convulsant Metrazole. This effect was time- and dose-dependent and was abolished by prior treatment with the anticonvulsant drugs diazepam or pentobarbital. About 60 minutes after administration of Metrazole, c-fos messenger RNA reached a maximum and declined to basal levels after 180 minutes. A further decrease below that in normal brain was observed before a return to basal levels after 16 hours. While Metrazole still elicited seizures during this period, reinduction of c-fos was largely refractory. At 90 minutes, c-fos protein was observed in the nuclei of neurons in the dentate gyrus, and in the pyriform and cingulate cortices. Subsequently, c-fos protein appeared throughout the cortex, hippocampus, and limbic system. Thus, seizure activity results in increased c-fos gene expression in particular subsets of neurons.