Forced rather than voluntary exercise entrains peripheral clocks via a corticosterone/noradrenaline increase in PER2::LUC mice.
Forced rather than voluntary exercise entrains peripheral clocks via a corticosterone/noradrenaline increase in PER2::LUC mice.
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DOI：
10.1038/srep27607
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发表时间：
2016-06-08
影响因子：
4.6
通讯作者：
Shibata S
中科院分区：
文献类型：
--
作者：
Sasaki H;Hattori Y;Ikeda Y;Kamagata M;Iwami S;Yasuda S;Tahara Y;Shibata S
在非活动期的运动可以诱导小鼠的自发活动和外周生物钟节律，然而，这种诱导的机制尚未阐明。在这里，我们发现，在PER2：：LUC小鼠外周时钟的生物发光节律强烈夹带强制跑步机和强制轮运行的运动，而不是自愿轮运行的运动在中间时间在不活动期间。运动诱导的夹带伴随着外周组织中血清皮质酮和去甲肾上腺素水平的增加，类似于物理应激诱导的反应。肾上腺切除术与去甲肾上腺素受体阻滞剂完全阻断了跑步机运动诱导的夹带。运动对外周生物钟的影响与哺乳动物的主要振荡器视交叉上核生物钟无关。本研究结果表明，强迫运动的反应，而不是自愿的运动，可能类似于应激，并通过激活肾上腺和交感神经系统的外周时钟的诱导能力。
Exercise during the inactive period can entrain locomotor activity and peripheral circadian clock rhythm in mice; however, mechanisms underlying this entrainment are yet to be elucidated. Here, we showed that the bioluminescence rhythm of peripheral clocks in PER2::LUC mice was strongly entrained by forced treadmill and forced wheel-running exercise rather than by voluntary wheel-running exercise at middle time during the inactivity period. Exercise-induced entrainment was accompanied by increased levels of serum corticosterone and norepinephrine in peripheral tissues, similar to the physical stress-induced response. Adrenalectomy with norepinephrine receptor blockers completely blocked the treadmill exercise-induced entrainment. The entrainment of the peripheral clock by exercise is independent of the suprachiasmatic nucleus clock, the main oscillator in mammals. The present results suggest that the response of forced exercise, but not voluntary exercise, may be similar to that of stress, and possesses the entrainment ability of peripheral clocks through the activation of the adrenal gland and the sympathetic nervous system.