SURVIVAL AND RENEWAL OF MURINE STEM SPERMATOGONIA FOLLOWING CO-60 GAMMA-RADIATION
SURVIVAL AND RENEWAL OF MURINE STEM SPERMATOGONIA FOLLOWING CO-60 GAMMA-RADIATION
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DOI：
10.2307/3575597
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
ERICKSON, BH
中科院分区：
文献类型：
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作者：
ERICKSON, BH
分离的、成对的和克隆的精原细胞在全部安装的曲细精管中观察时最可靠。在25-800拉德的照射剂量和1-14天的间隔后，从80-90天龄的BALB/c小鼠的睾丸中分离管状节段。无论是孤立的，也不是成对的精原细胞明显受到影响的剂量高达200拉德，和孤立的精原细胞的特点是由D 0的288拉德和3.8的n。与此相反，人口的A1精原细胞克隆显着减少的剂量为25拉德。克隆的D 0和n分别为217 rad和0.5。在明显的情况下，在200拉德的剂量和更少的坏死显著升高，A1克隆的损失归因于有丝分裂抑制。返回的克隆种群的照射前的水平显着的异步克隆间分化的增加和有丝分裂活动的增加内的三个精原细胞类，这是特别是这样的克隆。分离的精原细胞对辐射的反应性相对较低，与克隆的高辐射敏感性和有丝分裂活性相比，它的有丝分裂率较低，而且它明显具有切除异常细胞和产生额外克隆的能力，这导致了初步的结论，即克隆，而不是分离的精原细胞，是干细胞。
Isolated, paired and clonal spermatogonia are distinguished most reliably when viewed in segments of seminiferous tubules mounted in toto. Tubular segments were isolated from the testes of 80-90 day old BALB/c mice following irradiation doses of 25-800 rad and intervals of 1-14 days. Neither isolated nor paired spermatogonia were apparently affected by doses up to 200 rad, and the isolated spermatogonium was characterized by D0 of 288 rad and an n of 3.8. In contrast the population of A1-spermatogonial clones was markedly reduced by a dose of 25 rad. The D0 and n for the clone were 217 rad and 0.5, respectively. In the apparent absence of a significant elevation in necrosis at doses of 200 rad and less, the loss of A1 clones was attributed to mitotic inhibition. Return of the clonal population to the preirradiation level was marked by an increase in asynchronous interclonal differentiation and an increase in mitotic activity within the three spermatogonial classes, this being especially so with respect to the clone. The comparative unresponsiveness of the isolated spermatogonium to irradiation, its low mitotic rate when contrasted with the clone''s high radiosensitivity and mitotic activity, and its apparent ability to excise an abnormal cell and otherwise fragment to give rise to additional clones led to the tentative conclusion that the clone, and not the isolated spermatogonium, is the stem cell.