Free and Total Glycerin in Biodiesel: Accurate Quantitation by Easy Ambient Sonic-Spray Ionization Mass Spectrometry
Free and Total Glycerin in Biodiesel: Accurate Quantitation by Easy Ambient Sonic-Spray Ionization Mass Spectrometry
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DOI：
10.1021/ef300399m
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发表时间：
2012-05-01
期刊：
影响因子：
5.3
通讯作者：
Alberici, Rosana M.
中科院分区：
文献类型：
--
作者：
Fernandes, Anna Maria A. P.;Tega, David U.;Alberici, Rosana M.
生物柴油中游离和总甘油含量的定量是通过一个简单而直接的环境解吸/电离质谱技术进行的。简单的环境声喷雾电离质谱法（EASI-MS）适用于粗品或衍生样品加标内标，允许立即定量游离和总甘油，生物柴油的主要质量参数。通过EASI-MS获得的游离甘油的线性范围为0.005至0.100%（m/m），结合甘油（单-、二-和三酰基甘油酯）的线性范围为0.01至0.50%（m/m）。这些值与主要监管机构对生物柴油质量控制的要求一致，对于游离甘油，最大值为0.02%（m/m），对于总甘油，最大值为0.25%（m/m）。简单快速的EASI（+）-MS方案似乎为要求更高、更耗时的气相色谱-火焰离子化检测（GC-FID）标准方法提供了适当的替代或替代方案。
Quantitation of free and total glycerin content in biodiesel is performed via a simple and direct ambient desorption/ionization mass spectrometric technique. Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is applied to either the crude or derivatized sample spiked with an internal standard allowing for immediate quantitation of free and total glycerin, a major quality parameter for biodiesel. The linear range obtained by EASI-MS for free glycerin was from 0.005 to 0.100% (m/m), and that for bound glycerin (mono-, di-, and triacylglycerides) was from 0.01 to 0.50% (m/m). These values are in concordance with those required by major regulatory agencies for biodiesel quality control, which are, for free glycerin, a 0.02% (m/m) maximum and, for total glycerin, a 0.25% (m/m) maximum. The simple and fast EASI(+)-MS protocol seems to offer a proper substitute or alternative for the more demanding and time-consuming gas chromatography with flame ionization detection (GC-FID) standard methodology.