EFFICIENT CLEAVAGE OF P220 BY POLIOVIRUS 2A(PRO) EXPRESSION IN MAMMALIAN-CELLS - EFFECTS ON VACCINA-VIRUS
EFFICIENT CLEAVAGE OF P220 BY POLIOVIRUS 2A(PRO) EXPRESSION IN MAMMALIAN-CELLS - EFFECTS ON VACCINA-VIRUS
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DOI：
10.1006/bbrc.1995.2553
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发表时间：
1995-10-24
影响因子：
3.1
通讯作者：
CARRASCO, L
中科院分区：
文献类型：
--
作者：
ALDABE, R;FEDUCHI, E;CARRASCO, L
脊髓灰质炎病毒蛋白酶 2A 裂解 p220，它是起始因子 eIF-4F 的一个组成部分。已经产生了识别 p220 和来自不同物种的切割产物的多克隆抗体。用克隆在不同质粒中的脊髓灰质炎病毒 2A(pro) 转染几种细胞系，在感染 VT7（一种表达 T7 RNA 聚合酶的重组痘苗病毒）后，可有效裂解 p220。在这些条件下，痘苗病毒蛋白的合成受到严重抑制，而来自类似质粒的脊髓灰质炎病毒蛋白2C的表达则没有影响。这些结果首次显示了 p220 裂解对受感染细胞中痘苗病毒翻译的影响。 (C) 1995 学术出版社
Poliovirus protease 2A cleaves p220, a component of initiation factor eIF-4F. Polyclonal antibodies that recognize p220 and the cleaved products from different species have been raised. Transfection of several cell lines with poliovirus 2A(pro) cloned in different plasmids leads to efficient cleavage of p220 upon infection with VT7, a recombinant vaccinia virus that expresses the T7 RNA polymerase. Under these conditions vaccinia virus protein synthesis is severely inhibited, while expression of poliovirus protein 2C from a similar plasmid has no effect. These results show by the first time the effects of p220 cleavage on vaccinia virus translation in the infected cells. (C) 1995 Academic Press, Inc.