Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules
Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules
复制标题
量子点和氧化石墨烯之间的荧光共振能量转移用于传感生物分子
DOI：
10.1021/ac100852z
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发表时间：
2010-07-01
影响因子：
7.4
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
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作者：
Dong, Haifeng;Gao, Wenchao;Ju, Huangxian
本工作设计了一种新的平台，通过从量子点（QD）到氧化石墨烯（GO）的荧光共振能量转移（FRET）来有效地传感生物分子。首先用分子信标（MB）作为探针对量子点进行修饰以识别目标分析物。MB和GO之间的强相互作用导致量子点的荧光猝灭。在识别靶标后，量子点和GO之间的距离增加，并且靶标结合的MB和GO之间的相互作用变弱，这显著阻碍了FRET，从而增加了量子点的荧光。荧光强度的变化产生了一种检测目标的新方法。GO-猝灭法可用于DNA序列的检测，具有合成MB荧光探针劳动量少、猝灭效率高、灵敏度高、特异性好等优点。通过用适配体取代MB，该策略可以方便地扩展到其他生物分子的检测，这已经被适配体与蛋白质之间的相互作用所证明。据我们所知，这是量子点和GO之间的FRET的第一个应用程序，并为生物识别事件的灵敏检测开辟了新的机会。
This work designed a novel platform for effective sensing of biomolecules by fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to graphene oxide (GO). The QDs were first modified with a molecular beacon (MB) as a probe to recognize the target analyte. The strong interaction between MB and GO led to the fluorescent quenching of QDs. Upon the recognition of the target, the distance between the QDs and GO increased, and the interaction between target-bound MB and GO became weaker, which significantly hindered the FRET and, thus, increased the fluorescence of QDs. The change in fluorescent intensity produced a novel method for detection of the target. The GO-quenching approach could be used for detection of DNA sequences, with advantages such as less labor for synthesis of the MB-based fluorescent probe, high quenching efficiency and sensitivity, and good specificity. By substituting the MB with aptamer, this strategy could be conveniently extended for detection of other biomolecules, which had been demonstrated by the interaction between aptamer and protein. To the best of our knowledge, this is the first application of the FRET between QDs and GO and opens new opportunities for sensitive detection of biorecognition events.