Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy.
Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy.
复制标题
使用光学相干相位显微镜实时、无创地测量细胞机械行为。
DOI：
10.1016/j.ymeth.2017.10.010
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Gillies D
中科院分区：
文献类型：
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作者：
Gillies D
细胞的力学行为越来越被认为是癌症和干细胞研究中的核心生物物理参数，因此需要研究其力学行为的方法。我们开发了一种基于定量相位成像的新的定性方法，该方法能够使用光学相干相位显微镜(OCPM)在细胞分辨率下实时研究细胞的力学行为，并使用流体静压以非侵入性的方式刺激细胞。该方法被用来区分具有不同机械特性的细胞和由细胞松弛素D引起的瞬时变化。我们展示了定量相位成像的潜力，以检测由微流体通道中不同的静水压力引起的纳米级细胞内位移，反映细胞的机械行为。需要进一步的物理模拟才能得到定量的机械性能。
Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed.We have developed a novel qualitative method based on quantitative phase imaging which is capable of investigating cell mechanical behaviour in real-time at cellular resolution using optical coherence phase microscopy (OCPM), and stimulating the cells non-invasively using hydrostatic pressure. The method was exemplified to distinguish between cells with distinct mechanical properties, and transient change induced by Cytochalasin D.We showed the potential of quantitative phase imaging to detect nanoscale intracellular displacement induced by varying hydrostatic pressure in microfluidic channels, reflecting cell mechanical behaviour. Further physical modelling is required to yield quantitative mechanical properties.