Identification of novel striatal genes by expression profiling in adult mouse brain
Identification of novel striatal genes by expression profiling in adult mouse brain
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DOI：
10.1016/j.neuroscience.2007.02.040
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发表时间：
2007-05-25
期刊：
影响因子：
3.3
通讯作者：
Kieffer, B. L.
中科院分区：
文献类型：
--
作者：
Ghate, A.;Befort, K.;Kieffer, B. L.
大脑中的大规模转录组分析是识别对理解大脑组织和功能具有潜在兴趣的新基因的有力方法。我们利用微阵列技术测量了小鼠海马、额叶皮质和纹状体中约24，000个基因和表达序列标签的表达水平。使用从全脑获得的表达谱作为参考，我们将基因分为富集或限制于三个感兴趣区域之一的基因组。我们发现每个目标区域都有丰富的基因。此外，我们确定了14个基因的类别中的基因限制到纹状体，其中孤儿G蛋白偶联受体GPR 88和视黄酸受体β。这两个基因已经被报道在纹状体中选择性表达，从而验证了我们的实验方法。我们选择了6个纹状体限制性基因，以及10个纹状体丰富的候选人，这是以前未描述的。我们通过原位杂交分析和定量RT-PCR分析了它们在脑和外周器官中的表达。这些未知基因中有两个显示出显著的表达模式。纹状体限制性基因H3076 B11在整个纹状体中表现出均匀的表达，并且在纹状体中表现出独特的表达，代表了真正的纹状体标记。纹状体富集基因4833421 E05 Rik优先在吻侧纹状体中表达，并且在肾、肝和肺中也丰富。这两个基因可能有助于许多纹状体控制的行为，包括运动的启动，习惯的形成，或奖励和动机。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Large-scale transcriptome analysis in the brain is a powerful approach to identify novel genes of potential interest toward understanding cerebral organization and function. We utilized the microarray technology to measure expression levels of about 24,000 genes and expressed sequence tags in mouse hippocampus, frontal cortex and striatum. Using expression profile obtained from whole brain as a reference, we categorized the genes into groups of genes either enriched in, or restricted to, one of the three areas of interest. We found enriched genes for each target area. Further, we identified 14 genes in the category of genes restricted to the striatum, among which were the orphan G protein-coupled receptor GPR88 and retinoic acid receptor-beta. These two genes were already reported to be selectively expressed in the striatum, thus validating our experimental approach. We selected 6 striatal-restricted genes, as well as 10 striatal-enriched candidates, that were previously undescribed. We analyzed their expression by in situ hybridization analysis in the brain, and quantitative RT-PCR in both brain and peripheral organs. Two of these unknown genes displayed a notable expression pattern. The striatal-restricted gene H3076B11 shows uniform expression throughout and uniquely in the striatum, representing a genuine striatal marker. The striatal-enriched gene 4833421E05Rik is preferentially expressed in the rostral striatum, and is also abundant in kidney, liver and lung. These two genes may contribute to some of the many striatal-controlled behaviors, including initiation of movement, habit formation, or reward and motivation. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.