THE ISOLATION OF CAMPYLOBACTER-JEJUNI FROM CONTAMINATED SURFACES AND ITS SURVIVAL IN DILUENTS

THE ISOLATION OF CAMPYLOBACTER-JEJUNI FROM CONTAMINATED SURFACES AND ITS SURVIVAL IN DILUENTS
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DOI:
10.1016/0168-1605(94)00135-s
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发表时间:
1995-08-01
影响因子:
5.4
通讯作者:
MARTIN, K
MARTIN, K
中科院分区:
农林科学1区
文献类型:
--
作者:
HUMPHREY, T;MASON, M;MARTIN, K

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如果将拭子直接置于选择性培养基中而不是在培养前储存在稀释液中,则弯曲杆菌从污染表面的分离率得到提高。储存在稀释剂中导致活力的损失和剩余的活弯曲杆菌细胞往往亚致死损伤,这使他们敏感的选择剂在培养基中,并降低隔离rate.Campylobacter jejuni,悬浮在小血滴,是能够延长生存在工作表面上,如果滴仍然是液体,但细菌迅速死亡,一旦滴干。
The isolation rates of campylobacters from contaminated surfaces were improved if swabs were placed directly into selective media rather than being stored in diluents before culture. Storage in diluents resulted in a loss of viability and the remaining viable campylobacter cells were often sub-lethally injured which sensitised them to selective agents in culture media and reduced isolation rates.Campylobacter jejuni, suspended in small drops of blood, was capable of prolonged survival on work surfaces if the drops remained liquid but the bacterium died rapidly once drops had dried.