Polo-like kinase 1 (PLK1) is overexpressed in primary colorectal cancers
Polo-like kinase 1 (PLK1) is overexpressed in primary colorectal cancers
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DOI：
10.1111/j.1349-7006.2003.tb01411.x
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发表时间：
2003-02-01
期刊：
影响因子：
5.7
通讯作者：
Saji, S
中科院分区：
文献类型：
--
作者：
Takahashi, T;Sano, B;Saji, S
PLK （polo样激酶），是黑ogaster果蝇中的polo和酿酒酵母中的CDC5的人类对应物，属于一个丝氨酸/苏氨酸激酶家族。在有丝分裂过程中，它密切参与纺锤体形成和染色体分离。本研究的目的是确定与正常结肠粘膜相比，PLK1在原发性结直肠癌标本中是否过表达，并评估其与其他激酶的关系，作为一种潜在的新肿瘤标志物。在本研究中，我们对78例原发性结直肠癌和15例正常结直肠癌标本进行了PLK1表达的免疫组织化学分析。此外，我们还检测了其他激酶，Aurora-A和Aurora-C与PLK1表达的关系。在正常结肠黏膜中，15例中有13例隐窝细胞PLK1呈弱阳性，其余为阴性。PLK1在57例（73.1%）结直肠癌中表达升高，与pT（原发肿瘤侵袭）（P=0.0006， Mann-Whitney U检验）、pN（区域淋巴结）（P=0.008, chi(2)检验）和Dukes分级（P=0.0005， Mann-Whitney U检验）有显著的统计学意义。平均增殖细胞核抗原标记指数为52.3%，范围为24.1% ~ 77.3%。PLK1高表达和低表达病变值分别为54.7+/-10.3%（平均值+/-SD）和45.9+/-11.9% （P=0.002, Student's t检验）。PLK1与Aurora-A有显著相关性，但与Aurora-A或Aurora-C相比，PLK1的染色更为弥漫性和广泛性。有趣的是，PLK1过表达与结直肠癌中p53的积累显著相关。我们的研究结果表明，PLK1的过表达可能具有致病、预后和增殖的重要性，因此该激酶可能有潜力作为结直肠癌的新肿瘤标志物。[j] .中国科学（英文版）；2003;29(1):1 - 4。
PLK (polo-like kinase), the human counterpart of polo in Drosophila melanogaster and of CDC5 in Saccharomyces cerevisiae, belongs to a family of serine/threonine kinases. It is intimately involved in spindle formation and chromosome segregation during mitosis. The purpose of this study was to determine whether PLK1 is overexpressed in primary colorectal cancer specimens as compared with normal colon mucosa and to assess its relation to other kinases as a potential new tumor marker. In the present study, immunohistochemical analyses were performed of PLK1 expression in 78 primary colorectal cancers as well as 15 normal colorectal specimens. Furthermore, we examined the relationship between other kinases, Aurora-A and Aurora-C, and PLK1 expression. In normal colon mucosa, some crypt cells showed weakly positive staining for PLK1 in 13 out of 15 cases, the remaining cases being negative. Elevated expression of PLK1 was observed in 57 (73.1%) of the colorectal cancers, statistically significant associations being evident with pT (primary tumor invasion) (P=0.0006, Mann-Whitney U test), pN (regional lymph nodes) (P=0.008, chi(2) test) and the Dukes' classification (P=0.0005, Mann-Whitney U test). Mean proliferating cell nuclear antigen-labeling index was 52.3%, with a range of 24.1% to 77.3%. Values for lesions with high and low PLK1 expression were 54.7+/-10.3% (mean+/-SD) and 45.9+/-11.9% (P=0.002, Student's t test). PLK1 was significantly associated with Aurora-A, but PLK1 staining was more diffuse and extensive than for Aurora-A or Aurora-C. Interestingly, PLK1 overexpression was significantly associated with p53 accumulation in colorectal cancers. Our results suggest overexpression of PLK1 might be of pathogenic, prognostic and proliferative importance, so that this kinase might have potential as a new tumor marker for colorectal cancers. (Cancer Sci 2003; 94: 148-152).