Macrophages recognize size and shape of their targets.
Macrophages recognize size and shape of their targets.
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DOI：
10.1371/journal.pone.0010051
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发表时间：
2010-04-06
期刊：
影响因子：
3.7
通讯作者：
Mitragotri S
中科院分区：
文献类型：
--
作者：
Doshi N;Mitragotri S
巨噬细胞的识别是产生针对入侵病原体的免疫应答的关键过程。以前的研究集中在通过巨噬细胞表面的表面受体识别病原体。在这里，使用不同的几何形状的聚合物颗粒，代表各种细菌的大小和形状范围，目标的几何形状在识别的重要性进行了研究。本文报道的研究表明，不同几何形状的颗粒附着在巨噬细胞上表现出对大小和形状的强烈依赖性。对于所研究的所有尺寸和形状，具有2-3 µm范围内的最长尺寸的颗粒表现出最高的附着力。这也恰好是自然界中最常见的细菌的大小范围。巨噬细胞的表面特征，特别是膜皱褶，可能在巨噬细胞的这种基于几何形状的靶识别中发挥重要作用。这些发现对理解细菌的致病性和设计药物递送载体具有重要意义。
Recognition by macrophages is a key process in generating immune response against invading pathogens. Previous studies have focused on recognition of pathogens through surface receptors present on the macrophage's surface. Here, using polymeric particles of different geometries that represent the size and shape range of a variety of bacteria, the importance of target geometry in recognition was investigated. The studies reported here reveal that attachment of particles of different geometries to macrophages exhibits a strong dependence on size and shape. For all sizes and shapes studied, particles possessing the longest dimension in the range of 2–3 µm exhibited highest attachment. This also happens to be the size range of most commonly found bacteria in nature. The surface features of macrophages, in particular the membrane ruffles, might play an important role in this geometry-based target recognition by macrophages. These findings have significant implications in understanding the pathogenicity of bacteria and in designing drug delivery carriers.