Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer
Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer
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microRNA-21、maspin和血管内皮生长因子-C在膀胱癌中的表达及临床意义
DOI：
10.3892/ol.2015.3540
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发表时间：
2015-10-01
期刊：
影响因子：
2.9
通讯作者：
Chen, Min-Feng
中科院分区：
文献类型：
--
作者：
Zhang, Hui-Hui;Qi, Fan;Chen, Min-Feng
本研究旨在探讨microRNA-21（miR-21）、maspin和血管内皮生长因子C（VEGF-C）在膀胱癌中的表达及其临床意义。共收集了53个BC样本和12个正常膀胱组织样本。提取总信使RNA（mRNA），采用逆转录-定量聚合酶链反应（RT-qPCR）检测miR-21和maspin在膀胱癌和正常膀胱组织中的表达。免疫组化法检测maspin和VEGF-C蛋白的表达。此外，这些分子和某些临床病理参数之间的相关性进行了研究，并进行生存分析，以评估其预后意义。膀胱癌组织中miR-21 mRNA和VEGF-C蛋白的表达均高于正常膀胱组织，而maspin mRNA和蛋白的表达均低于正常膀胱组织（P <0.01）。miR-21、maspin和VEGF-C的表达与乳腺癌的分期、分级和淋巴结转移有关（P <0.05），而与其他临床病理特征无关。miR-21和maspin的mRNA表达呈负相关，相关系数为-0.978（P <0.001）。maspin和VEGF-C蛋白表达呈负相关，相关系数为-0.589（P <0.001）。miR-21和VEGF-C的过表达以及maspin表达的降低与较差的预后相关。这些结果表明，miR-21的上调、maspin表达的降低和VEGF-C的增强可能促进BC中的肿瘤进展。因此，miR-21、maspin和VEGF-C可能作为预后的生物标志物和BC的治疗靶点具有重要作用。
The present study aimed to explore the expression and clinical significance of microRNA-21 (miR-21), maspin and vascular endothelial growth factor C (VEGF-C) in bladder cancer (BC). A total of 53 BC samples and 12 normal bladder tissue samples were collected. Total messenger RNA (mRNA) was extracted, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to evaluate the expression of miR-21 and maspin in BC and normal bladder tissues. Immunohistochemistry was used for the detection of maspin and VEGF-C protein expression. Furthermore, the correlations between these molecules and certain clinicopathological parameters were investigated, and survival analysis was performed to assess their prognostic significance. miR-21 mRNA expression and VEGF-C protein expression were increased in BC tissues compared with those in normal bladder tissues, whereas maspin mRNA and protein expression levels in BC tissues were significantly decreased (P<0.01). miR-21, maspin and VEGF-C expression were significantly associated with the stage, grade and lymph node metastasis of BC (P<0.05), but not the other clinicopathological features evaluated. There was a marked inverse correlation between the mRNA expression of miR-21 and maspin, with a coefficient of -0.978 (P<0.001). Similarly, there was a significant inverse correlation between the protein expression of maspin and VEGF-C, with a coefficient of -0.589 (P<0.001). Overexpression of miR-21 and VEGF-C, as well as decreased maspin expression, were associated with a poorer prognosis. These results suggested that upregulation of miR-21, decreased maspin expression and enhanced VEGF-C in BC may promote tumor progression. miR-21, maspin and VEGF-C may therefore have significant roles as biomarkers for prognosis and as therapeutic targets of BC.