Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife.
Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife.
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DOI：
10.3390/tropicalmed2030031
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发表时间：
2017-07-21
影响因子：
2.9
通讯作者：
Müller T
中科院分区：
文献类型：
--
作者：
Moore SM;Gilbert A;Vos A;Freuling CM;Ellis C;Kliemt J;Müller T
细胞介导的和体液免疫效应在对抗狂犬病感染中都很重要，尽管体液免疫反应在狂犬病预防方面受到更多关注。几十年来，野生动物宿主种群的口服狂犬病疫苗（ORV）策略一直采用预防性接种的原则，以控制狂犬病病毒在散养宿主中的传播。关于狂犬病抗体的水平（以及测量它们的检测方法），对狂犬病病毒的抵抗力仍然存在很大的争论。在本文中，数据从已发表的文献和我们自己的未发表的动物研究狂犬病结合和中和抗体的诱导后，口服免疫动物减毒活疫苗或重组狂犬病疫苗，检查保护对致死性狂犬病感染的圈养挑战设置的相关性。我们的研究分析表明，尽管血清中和试验结果预期反映体内保护，但第28天的阻断酶联免疫吸附试验（ELISA）结果是更好的生存预测因子。ELISA试剂盒可能具有更高的精确度和基于试剂盒格式的固有标准化在不同研究和实验室之间比较结果的能力的优点。本文探讨了目前的知识和研究结果，以指导有意义的解释血清学结果在口服诱饵监测。
Both cell-mediated and humoral immune effectors are important in combating rabies infection, although the humoral response receives greater attention regarding rabies prevention. The principle of preventive vaccination has been adopted for strategies of oral rabies vaccination (ORV) of wildlife reservoir populations for decades to control circulation of rabies virus in free-ranging hosts. There remains much debate about the levels of rabies antibodies (and the assays to measure them) that confer resistance to rabies virus. In this paper, data from published literature and our own unpublished animal studies on the induction of rabies binding and neutralizing antibodies following oral immunization of animals with live attenuated or recombinant rabies vaccines, are examined as correlates of protection against lethal rabies infection in captive challenge settings. Analysis of our studies suggests that, though serum neutralization test results are expected to reflect in vivo protection, the blocking enzyme linked immunosorbent assay (ELISA) result at Day 28 was a better predictor of survival. ELISA kits may have an advantage of greater precision and ability to compare results among different studies and laboratories based on the inherent standardization of the kit format. This paper examines current knowledge and study findings to guide meaningful interpretation of serology results in oral baiting monitoring.