Comparative analysis of chloroplast DNA variability in wild and cultivated Citrullus species
Comparative analysis of chloroplast DNA variability in wild and cultivated Citrullus species
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DOI：
10.1007/s00122-003-1512-9
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发表时间：
2004-03-01
影响因子：
5.4
通讯作者：
Bakhtiyarova, R
中科院分区：
文献类型：
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作者：
Dane, F;Lang, P;Bakhtiyarova, R
利用叶绿体DNA的PCR扩增和限制性酶切位点分析，研究了西瓜属植物的种间和种内差异。超过55 C。lanatus和15 C.不同地理区域采集的colocynthis种质，C. ecirrhosus和C.使用的是小米。西瓜属中的大多数cpDNA变异是大插入缺失和转换与颠换的结果。ndhA、trnS-trnfM和trnC-trnD区域的插入缺失和限制性酶切位点处的几个取代可用于分离C.从其他西瓜属物种中分离出来。ndhF基因3'侧翼区限制性内切酶位点的核苷酸替换提供了C.毛状变种lanatus，栽培西瓜。类似地，在trnC-trnD区域的基因间间隔区的核苷酸取代导致了香橼的诊断单倍型，C。毛状变种柠檬酸盐。几个C。毛状变种Citroides种质表现出var.羊毛状单倍型C.与C.毛状变种除了在cpSSR位点处的独特插入之外，它们都是类柠檬酸盐。自从C. ecirrhosus缺乏C. lanatus，它可能是栽培西瓜的祖先。在C. colocynthis与地理来源有关。
PCR amplification and restriction site analysis of chloroplast (cp) DNA regions was used to detect inter- and intraspecific differences in the genus Citrullus. More than 55 C. lanatus and 15 C. colocynthis accessions collected from diverse geographical areas, C. ecirrhosus and C. rehmii were used. Most of the cpDNA variation within Citrullus was the result of large indels and transitions and transversions. Indels at the ndhA, trnS-trnfM and trnC-trnD regions and several substitutions at restriction enzyme sites can be used to separate C. colocynthis from the other Citrullus species. A nucleotide substitution at a restriction enzyme site at the 3' flanking region of ndhF provided a diagnostic haplotype for C. lanatus var. lanatus, the cultivated watermelon. Similarly, a nucleotide substitution at an intergenic spacer region of the trnC-trnD region resulted in a diagnostic haplotype for citron, C. lanatus var. citroides. Several C. lanatus var. citroides accessions showed the var. lanatus haplotype. C. rehmii showed almost the same haplotype as C. lanatus var. citroides with the exception of a unique insertion at a cpSSR site. Since C. ecirrhosus lacks the derived diagnostic nucleotide substitutions of C. lanatus, it is probably the progenitor of the cultivated watermelon. Intraspecific haplotypes detected within C. colocynthis were associated with geographic origin.