Isolation and application of a thermotolerant nitrifying bacterium Gordonia paraffinivorans N52 to sewage sludge composting for reducing nitrogen loss
Isolation and application of a thermotolerant nitrifying bacterium Gordonia paraffinivorans N52 to sewage sludge composting for reducing nitrogen loss
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耐热硝化细菌 Gordonia paraffinivorans N52 的分离及其在污泥堆肥中减少氮损失的应用
DOI：
10.1016/j.biortech.2022.127959
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发表时间：
2022
影响因子：
11.4
通讯作者：
Shumei Zhang
中科院分区：
文献类型：
--
作者：
Yi Zhao;Weiguang Li;Li Chen;Yujie Zhou;Liqiang Meng;Shumei Zhang
·分离到一株耐热异养硝化菌Gordonia paraffinivorans N52。·菌株N52在高温条件下具有高效的硝化能力。· N52接种提高了堆肥中硝酸盐含量，增强了硝化作用。·接种N52可减少污泥堆肥过程中的氨排放和氮损失。从污泥堆肥中分离得到一株具有异养硝化能力的耐高温菌株，经鉴定为Gordonia paraffinivorans N52。N52菌株在60℃下对铵态氮的利用率为51.8%，氮平衡结果表明，N52菌株消耗的铵态氮中有25.5%转化为硝化中间产物，53.0%转化为胞内氮，仅有5.2%流失。硝化相关酶的成功检测和功能基因的PCR扩增进一步证明了该菌株的硝化能力。正交试验结果表明，在C/N为15、温度为50℃、转速为150 rpm、pH为8的条件下，硝化效果最佳。与对照组相比，在污水污泥堆肥中添加Gordonia paraffinivorans N52可减少27.6%的氨排放量，加速NH 4 + -N向硝态氮的转化，减少总氮损失。因此，接种Gordonia paraffinivorans N52能有效控制堆肥过程中氨的排放，减少氮素的损失。
• A thermotolerant heterotrophic nitrifier Gordonia paraffinivorans N52 was isolated. • Strain N52 had efficient nitrification ability under high-temperature conditions. • N52 inoculation increased nitrate content and enhanced nitrification in composting. • N52 inoculation reduced ammonia emission and nitrogen loss in sludge composting. A thermotolerant strain with heterotrophic nitrification capability obtained from sludge composting was identified as Gordonia paraffinivorans N52. Strain N52 utilized 51.8% of ammonium nitrogen (NH 4 + -N) at 60℃, and the nitrogen balance results indicated that 25.5% of the consumed NH 4 + -N was changed into nitrification intermediates, 53.0% to intracellular nitrogen, and only 5.2% was lost. The successful detection of enzymes related to nitrification and PCR amplification of functional genes further demonstrated nitrification ability of the isolated strain. Moreover, orthogonal test indicated that conditions for the optimal nitrification performance were C/N 15, 50℃, 150 rpm and pH 8. Compared with the control group, the addition of Gordonia paraffinivorans N52 to sewage sludge composting reduced 27.6% of ammonia emissions, accelerated the conversion from NH 4 + -N to nitrate nitrogen and decreased the total nitrogen loss. These results suggested that inoculation of Gordonia paraffinivorans N52 effectively controlled ammonia emissions and reduced nitrogen loss in composting.