Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in Escherichia coli.
Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in Escherichia coli.
复制标题
易错聚合酶 DNA 聚合酶 IV 负责大肠杆菌适应性突变期间的短暂超突变。
DOI：
10.1128/jb.185.11.3469-3472.2003
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发表时间：
2003
影响因子：
3.2
通讯作者：
Foster,PatriciaL
中科院分区：
文献类型：
--
作者：
Tompkins,JoshuaD;Nelson,JenniferL;Hazel,JillC;Leugers,StacyL;Stumpf,JeffreyD;Foster,PatriciaL
比较了有和没有易错DNA聚合酶IV（Pol IV）的大肠杆菌适应性Lac+回复突变体中非选择性突变的频率。该频率在Pol IV缺陷型菌株中比野生型菌株低七倍以上。因此，在超突变期间发生的突变是由于Pol IV。
The frequencies of nonselected mutations among adaptive Lac+revertants ofEscherichia colistrains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.