Investigation of mammary blood flow changes by transrectal colour Doppler sonography in an Escherichia coli mastitis model

Investigation of mammary blood flow changes by transrectal colour Doppler sonography in an Escherichia coli mastitis model
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经直肠彩色多普勒超声检查大肠杆菌乳腺炎模型乳腺血流变化的研究

DOI:
10.1017/s0022029910000105
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发表时间:
2010
影响因子:
2.1
通讯作者:
Zerbe H
Zerbe H
中科院分区:
农林科学3区
文献类型:
--
作者:
Potapow A;Sauter-Louis C;Schmauder S;Friker J;Nautrup CP;Mehne D;Petzl W;Zerbe H

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本初步研究的目的是评估经直肠彩色多普勒超声(TCDS)确定实验诱导大肠杆菌乳腺炎的奶牛阴部上腹部干血流的可行性。对5头产犊后4-6个月的荷斯坦奶牛进行了两次试验。所有监测的乳房乳区最初临床健康,体细胞计数(SCC)<50 000个细胞/ml,细菌学阴性。用血流量(BFV)描述左右阴部上腹干的血流。在研究的方法学部分,评价了该方法的观察员内精密度。BFV的变异系数为7.1%(左侧)和9.4%(右侧)。左、右血管BFV的组内相关系数分别为0.99(P<0.001)和0.75(P= 0.004)。BFV在左侧和右侧之间、挤奶前和挤奶后之间、发情期和间情期之间没有显著差异。在本研究的实验部分中,0和12 h p.i. (post感染)(P=0·043),并在感染后12和24 h之间降低BFV。(P= 0.043)。在左右(对照-感染)比较中,在感染后12 h观察到右侧BFV显著增加。(P= 0.043)。在注射后0和12 h之间,SCC增加的差异与BFV增加的差异呈正相关。右侧感染侧(斯皮尔曼ρ=1·00; P=0·043)。结果表明,TCDS是一种可重复的技术,用于研究在急性乳腺炎的早期阶段的病理性乳腺血流变化。
The objectives of this preliminary investigation were to evaluate the feasibility of transrectal colour Doppler sonography (TCDS) for determining blood flow of the pudendoepigastric trunk in cows with experimentally induced Escherichia coli Mastitis. Five primiparous Holstein dairy cows, 4–6 months after calving, were examined in two trials. All monitored udder quarters were initially clinically healthy, somatic cell count (SCC) was <50 000 cells/ml and bacteriologically negative. The blood flow of the left and the right pudendoepigastric trunk was described by the blood flow volume (BFV). In the methodological part of the study, the intra-observer precision of the method was evaluated. The coefficients of variation of the BFV were 7·1% for the left and 9·4% for the right pudendoepigastric trunk. The intraclass correlation coefficients of the BFV were 0·99 (P<0·001) for the left and 0·75 (P=0·004) for the right vessel. BFV did not differ significantly between the left and the right side nor between pre- and post-milking nor between oestrus and dioestrus. In the experimental part of the study, significant differences of increasing BFV between 0 and 12 h p.i. (post infectionem) (P=0·043) and decreasing BFV between 12 and 24 h p.i. (P=0·043) were discovered for the pudendoepigastric trunk of the infected right side. In the left-right (control-infection) comparison a significant increase of the right BFV was observed at 12 h p.i. (P=0·043). The difference of an increasing SCC correlated positively with the difference of an increasing BFV between 0 and 12 h p.i. (Spearman's ρ=1·00; P=0·043) for the right infected side. It was shown that TCDS is a reproducible technique for investigating pathological mammary blood flow changes at an early stage of acute mastitis.
DOI: 10.1016/s0167-5877(01)00176-3
发表时间: 2001-04-13
影响因子: 2.6
作者:
de Mol, RM;Ouweltjes, W
通讯作者: Ouweltjes, W
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DOI: 10.1111/j.1740-8261.2000.tb01472.x
发表时间: 2000
期刊: Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association
影响因子: --
作者:
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通讯作者: H. Meier
DOI: --
发表时间: 2006
影响因子: 3.5
作者:
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通讯作者: J. Vanselow;Wei Yang;J. Herrmann;H. Zerbe;H. Schuberth;W. Petzl;W. Tomek;H. Seyfert
DOI: 10.1016/s0093-691x(98)00094-6
发表时间: 1998-06-01
期刊: THERIOGENOLOGY
影响因子: 2.8
作者:
Bollwein, H;Maierl, J;Stolla, R
通讯作者: Stolla, R
DOI: 10.1111/j.1740-8261.1999.tb01842.x
发表时间: 1999-01-01
影响因子: 1.7
作者:
Lamb, CR;Burton, CA;Carlisle, CH
通讯作者: Carlisle, CH