Levels of hypoxia-inducible factor-1α during breast carcinogenesis
Levels of hypoxia-inducible factor-1α during breast carcinogenesis
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DOI：
10.1093/jnci/93.4.309
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发表时间：
2001-02-21
影响因子：
10.3
通讯作者：
van der Wall, E
中科院分区：
文献类型：
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作者：
Bos, R;Zhong, H;van der Wall, E
背景资料：缺氧诱导因子-1（HIF-1）是一种转录因子，在肿瘤生长和转移的关键途径中调节基因表达。在这份报告中，我们研究了乳腺组织癌变过程中HIF-1 α的水平是否增加，以及是否与其他肿瘤生物标志物相关。方法：石蜡包埋的临床标本来自乳腺肿瘤发生的五个病理阶段和正常乳腺组织。HIF-1 α蛋白和生物标志物血管内皮生长因子（VEGF）、HER-2/neu、p53、Ki-67和雌激素受体（ER）通过化学方法鉴定，并测定微血管密度（血管生成的量度）。统计学检验HIF-1 α水平和这些生物标志物之间的关联。所有统计检验均为双侧检验。结果：HIF-1 α阳性细胞率随病理分期的增加而增加（P
Background: Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that regulates gene expression in critical pathways involved in tumor growth and metastases. In this report, we investigated whether the level of HIF-1 alpha is increased during carcinogenesis in breast tissue and is associated with other tumor biomarkers. Methods: Paraffin-emhedded clinical specimens from five pathologic stages of breast tumorigenesis and from normal breast tissue were used. HIF-1 alpha protein and the biomarkers vascular endothelial growth factor (VEGF), HER-2/neu, p53, Ki-67, and estrogen receptor (ER) were identified immunohistochemically, and microvessel density (a measure of angiogenesis) was determined, Associations among levels of HIF-1 alpha and these biomarkers were tested statistically. All statistical tests are two-sided. Results: The frequency of HIF-1 alpha -positive cells in a specimen increased with the specimen's pathologic stage (P