Techniques for In Vitro and In Vivo Fertilization in the Rat
Techniques for In Vitro and In Vivo Fertilization in the Rat
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DOI：
10.1007/978-1-60327-389-3_22
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Serikawa, Tadao
中科院分区：
文献类型：
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作者：
Kashiwazaki, Naomi;Seita, Yasunari;Serikawa, Tadao
尽管体外受精和体内受精是恢复保存的精子和储存在大鼠体内的雄性精子的强大工具，但这项技术已经逐渐变得重要起来。然而，这些技术并没有被广泛应用于高效生产老鼠后代，因为这些技术需要大量的技能。本章描述了大鼠体外受精和体内受精的方案。也就是说，提供了精子采集、精子冷冻保存、精子预孵化、体外受精的人工授精(与精子和卵母细胞共培养)和与新鲜或冷冻/解冻的精子进行体内受精的宫内受精。
Although in vitro and in vivo fertilization are powerful tools for restoring conserved sperm as well as stocked males in the rat, the techniques have progressively gained importance. However, the techniques are not used extensively for efficient production of rat offspring, because the techniques require a great deal of skill. This chapter describes the protocols for in vitro and in vivo fertilization in the rat. Namely, sperm collection, sperm cryopreservation, pre-incubation of sperm, and insemination (co-culture with sperm and oocytes) for in vitro fertilization and intrauterine insemination for in vivo fertilization with fresh or frozen/thawed spermatozoa are provided.