β-Bungarotoxin is a potent inducer of apoptosis in cultured rat neurons by receptor-mediated internalization
β-Bungarotoxin is a potent inducer of apoptosis in cultured rat neurons by receptor-mediated internalization
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DOI：
10.1046/j.0953-816x.2001.01699.x
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发表时间：
2001-09-01
影响因子：
3.4
通讯作者：
Becker, CM
中科院分区：
文献类型：
--
作者：
Herkert, M;Shakhman, O;Becker, CM
具有神经毒性的磷脂酶 A(2)，即 β-银环蛇毒素 (β-BuTx)，是台湾带状金环蛇 (Bungarus multicinctus) 蛇毒的成分。 beta-BuTx 影响神经肌肉接头的突触前神经末梢功能，并诱导整个哺乳动物和鸟类中枢神经系统广泛的神经元细胞死亡。为了分析β-BuTx介导的细胞死亡的初始事件，将毒素应用于培养的大鼠海马神经元，在24小时内以浓度依赖性方式（EC50 = 5 x 10(-13) M）诱导神经元细胞死亡。荧光标记的 β-BuTx 完全被神经元掺入
The neurotoxic phospholipase A(2), beta -bungarotoxin (beta -BuTx), is a component of the snake venom from the Taiwanese banded krait Bungarus multicinctus. beta -BuTx affects presynaptic nerve terminal function of the neuromuscular junction and induces widespread neuronal cell death throughout the mammalian and avian CNS. To analyse the initial events of beta -BuTx-mediated cell death, the toxin was applied to cultured rat hippocampal neurons where it induced neuronal cell death in a concentration-dependent manner (EC50 = 5 x 10(-13) M) within 24 h. Fluorescence labelled beta -BuTx was completely incorporated by neurons within