Selective Capture and Quick Detection of Targeting Cells with SERS-Coding Microsphere Suspension Chip
Selective Capture and Quick Detection of Targeting Cells with SERS-Coding Microsphere Suspension Chip
复制标题
使用 SERS 编码微球悬浮芯片选择性捕获和快速检测靶细胞
DOI：
10.1002/smll.201402531
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发表时间：
2015
期刊：
影响因子：
13.3
通讯作者：
Tang Chuanbing
中科院分区：
文献类型：
--
作者：
Li Dian;Zhang Yuting;Li Ruimin;Guo Jia;Wang Changchun;Tang Chuanbing
从血液中捕获的循环肿瘤细胞（CTC）代表复发性癌症和转移性病变，以监测癌症的情况。我们开发了表面增强拉曼散射（Sers）编码微球悬浮芯片，作为快速高效捕获、回收和检测靶向癌细胞的新策略。以HeLa细胞为模型CTC，首次利用叶酸作为识别分子，将其固定在磁性复合微球中，实现了对HeLa细胞的高捕获率（高达95%）。该复合微球利用二硫键作为壳层的交联剂和叶酸的连接基团，捕获细胞后，在谷胱甘肽溶液洗脱下，20 min内可回收90%的细胞。利用Sers的指纹特征，我们通过将Sers编码的微球悬浮芯片引入CTC，表征具有报告分子4-氨基苯硫酚的独特信号的捕获/回收的细胞。筛选细胞的探索性实验表明，磁性复合微球可以从混合细胞样品中选择性捕获HeLa细胞，表明磁性复合微球在真实的血液样品中具有捕获CTC的潜力。
Circulating tumor cells (CTCs) captured from blood fluid represent recurrent cancers and metastatic lesions to monitor the situation of cancers. We develop surface‐enhanced Raman scattering (SERS)‐coding microsphere suspension chip as a new strategy for fast and efficient capture, recovery, and detection of targeting cancer cells. Using HeLa cells as model CTCs, we first utilize folate as a recognition molecule to be immobilized in magnetic composite microspheres for capturing HeLa cells and attaining high capturing efficacy (up to 95%). After capturing cells, the composite microsphere, which utilizes a disulfide bond as crosslinker in the polymer shell and as a spacer for linking folate, can recycle 90% cells within 20 min eluted by glutathion solution. Taking advantage of the SERS with fingerprint features, we characterize captured/recovered cells with the unique signal of report‐molecule 4‐aminothiophenol through introducing the SERS‐coding microsphere suspension chip to CTCs. Finally, the exploratory experiment of sieving cells shows that the magnetic composite microspheres can selectively capture the HeLa cells from samples of mixed cells, indicating that these magnetic composite microspheres have potential in real blood samples for capturing CTCs.