Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function.
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DOI：
10.1039/b705358d
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发表时间：
2007-10
影响因子：
4.9
通讯作者：
Kouhei Shinoda;Takuji Hasegawa;Hiroaki Sato;M. Shinozaki;H. Kuramoto;Y. Takamiya;Tsutomu Sato;N. Nikaidou;Takeshi Watanabe;T. Hoshino
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文献类型：
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作者：
Kouhei Shinoda;Takuji Hasegawa;Hiroaki Sato;M. Shinozaki;H. Kuramoto;Y. Takamiya;Tsutomu Sato;N. Nikaidou;Takeshi Watanabe;T. Hoshino
由VioABDE混合酶产生的紫罗兰素的生物合成中间体是5-(5-hydroxy-1H-indol-3-yl)-3-(1H-indol-3-yl)-1H-pyrrole-2-carboxylic酸，命名为原紫罗兰酸，表明负责最终生物合成步骤的VIOC在右侧吲哚环的2位进行氧化，形成中心吡咯烷酮核心的氧化反应是以非酶方式进行的。
A biosynthetic intermediate of violacein produced by the mixed enzymes of VioABDE was elucidated to be 5-(5-hydroxy-1H-indol-3-yl)-3-(1H-indol-3-yl)-1H-pyrrole-2-carboxylic acid, named protoviolaceinic acid, indicating that VioC, responsible for the final biosynthetic step, works to oxygenate at the 2-position of the right side indole ring, and that the oxygenation reaction to form the central pyrrolidone core proceeds in a non-enzymatic fashion.