Isolation and air-liquid interface culture of human large airway and bronchiolar epithelial cells.
Isolation and air-liquid interface culture of human large airway and bronchiolar epithelial cells.
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DOI：
10.1016/j.jcf.2004.05.010
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Chinet, Thierry
中科院分区：
文献类型：
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作者：
Bals, Robert;Beisswenger, Christoph;Chinet, Thierry
本文介绍了从大气道和远端气道分离和培养人气道上皮细胞的技术。这两种细胞类型都是从手术期间收集的肺碎片中获得的。该方案开始于肺片的清洗和解剖的初始步骤，以将大气道或远端气道与周围的实质分离。第二步是从解剖的大气道或远端气道中酶促分离上皮细胞。然后通过离心收集细胞，然后接种到胶原蛋白表面上。上皮细胞可以在气液界面生长，通常在数天内形成融合的功能性上皮层。
This article describes the techniques of isolation and culture of human airway epithelial cells from large airways and from distal airways. Both cell types are obtained from lung pieces collected during surgery. The protocols start with an initial step of washing and dissection of the lung pieces to separate large airways or distal airways from the surrounding parenchyma. The second step is enzymatic isolation of epithelial cells from the dissected large or distal airways. Cells are then collected by centrifugation and then seeded onto collagen surfaces. Epithelial cells can be grown at an air-liquid interface and usually form a confluent and functional epithelial layer within days.