Quantitative long-term imaging of the functional representation of a whisker in rat barrel cortex.
Quantitative long-term imaging of the functional representation of a whisker in rat barrel cortex.
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大鼠桶状皮层胡须功能表征的定量长期成像。
DOI：
10.1073/pnas.93.10.4942
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发表时间：
1996
影响因子：
11.1
通讯作者：
Frostig,RD
中科院分区：
文献类型：
--
作者：
Masino,SA;Frostig,RD
在这项研究中，我们对大鼠桶状皮质中的内在信号进行了长期光学成像，并随着时间的推移重复量化单个胡须的功能表示。超过 1 个月的慢性成像的成功使得能够评估这种感觉表征的正常动态范围。在个体动物中，持续几周的时间，我们发现：（i）胡须 C2 的量化功能表征的平均空间范围出人意料地大——1.71 mm2（半高面积）； (ii) 职能代表的位置一致； (iii) 时空特征存在持续但非系统性的变化，例如函数表示的大小、形状和响应幅度。这些结果支持对桶状皮层功能组织的修改描述，其中虽然精确定位的模块对应于特定的胡须，但该模块是动态的、大的，并且与许多其他胡须的模块有相当大的重叠。
In this study, we implement chronic optical imaging of intrinsic signals in rat barrel cortex and repeatedly quantify the functional representation of a single whisker over time. The success of chronic imaging for more than 1 month enabled an evaluation of the normal dynamic range of this sensory representation. In individual animals for a period of several weeks, we found that: (i) the average spatial extent of the quantified functional representation of whisker C2 is surprisingly large--1.71 mm2 (area at half-height); (ii) the location of the functional representation is consistent; and (iii) there are ongoing but nonsystematic changes in spatiotemporal characteristics such as the size, shape, and response amplitude of the functional representation. These results support a modified description of the functional organization of barrel cortex, where although a precisely located module corresponds to a specific whisker, this module is dynamic, large, and overlaps considerably with the modules of many other whiskers.