Chronic restraint stress induces changes in synapse morphology in stratum lacunosum-moleculare CA1 rat hippocampus:: A stereological and three-dimensional ultrastructural study
Chronic restraint stress induces changes in synapse morphology in stratum lacunosum-moleculare CA1 rat hippocampus:: A stereological and three-dimensional ultrastructural study
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DOI：
10.1016/j.neuroscience.2006.02.072
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Stewart, M. G.
中科院分区：
文献类型：
--
作者：
Donohue, H. S.;Gabbott, P. L. A.;Stewart, M. G.
已知慢性束缚应激会影响海马的形态和突触组织，主要在CA 3内，但也在CA 1和齿状回内。在这项研究中，我们提供了第一个证据，具体的超微结构的改变影响不对称轴棘突触CA 1层腔隙-moleculare慢性束缚应激（6小时/天，21天）在大鼠。采用连续切片三维重建方法研究了对照组（n=4）和慢性束缚应激组（n=3）动物不对称轴棘突触后密度的结构。树突棘轮廓（棘头+颈部）与采样的突触接触（30每只动物）也重建在three-dimensions.Morphometric分析显示突触后密度表面积（+36%，P=0.03）和突触后密度体积（+79%，P=0.003）在慢性束缚应激组中的显着增加。这些变化直接与“非黄斑”（穿孔，复杂和分段）突触后密度。在慢性束缚应激组中也检测到“突触后密度表面积/棘表面积”比率的高度显著的总体增加（+27%; P=0.002）。相比之下，未发现组间脊柱参数的定量变化。骑士！方法观察慢性束缚应激对大鼠海马CA 1区体积的影响。在慢性束缚应激组中，总背侧前CA 1海马的平均体积显著较低（-16%，P=0.036）。然而，校正体积变化时，没有显着的变化，在相对估计的平均数量的不对称轴棘突触检测到在CA 1层腔隙分子之间的控制和慢性束缚应激组。这些数据表明，在大鼠CA 1层腔隙分子兴奋性轴棘突触连接的结构重塑作为慢性束缚应激的结果。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Chronic restraint stress is known to affect the morphology and synaptic organization of the hippocampus, predominantly within CA3 but also in CA1 and dentate gyrus. In this study, we provide the first evidence for specific ultrastructural alterations affecting asymmetric axo-spinous synapses in CA1 stratum lacunosum-moleculare following chronic restraint stress (6 h/day, 21 days) in the rat. The structure of asymmetric axo-spinous post-synaptic densities was investigated using serial section three-dimensional reconstruction procedures in control (n=4) and chronic restraint stress (n=3) animals. Dendritic spine profiles (spine head+neck) associated with the sampled synaptic contacts (30 per animal) were also reconstructed in three-dimensions.Morphometric analyses revealed a significant increase in post-synaptic density surface area (+36%; P=0.03) and a highly significant increase in post-synaptic density volume (+79%; P=0.003) in the chronic restraint stress group. These changes were directly associated with 'non-macular' (perforated, complex and segmented) post-synaptic densities. A highly significant overall increase in the 'post-synaptic density surface area/spine surface area' ratio was also detected in the chronic restraint stress group (+27%; P=0.002). In contrast, no quantitative changes in spine parameters were found between groups. The Cavalier! method was used to assess the effects of chronic restraint stress exposure upon CA1 hippocampal volume. The mean volume of total dorsal anterior CA1 hippocampus was significantly lower in the chronic restraint stress group (-16%; P=0.036). However, when corrected for volume changes, no significant alteration in a relative estimate of the mean number of asymmetric axo-spinous synapses was detected in CA1 stratum lacunosum-moleculare between control and chronic restraint stress groups. The data indicate a structural remodeling of excitatory axo-spinous synaptic connectivity in rat CA1 stratum lacunosum-moleculare as a result of chronic restraint stress. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.