Rhesus monkey (Macaca mulatta) mucosal antimicrobial peptides are close homologues of human molecules
Rhesus monkey (Macaca mulatta) mucosal antimicrobial peptides are close homologues of human molecules
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DOI：
10.1128/cdli.8.2.370-375.2001
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发表时间：
2001-03-01
期刊：
影响因子：
--
通讯作者：
Wilson, JM
中科院分区：
文献类型：
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作者：
Bals, R;Lang, C;Wilson, JM
粘膜表面宿主防御的一个组成部分似乎是上皮来源的抗菌肽。防御素和抗菌素家族的分子已经在包括人类和小鼠在内的几个物种中进行了研究。我们在这篇报告中描述了恒河猴人类粘膜抗菌肽同源物的鉴定和表征。我们利用逆转录酶PCR方法克隆了恒河猴β -防御素1和2 （rhBD-1和rhBD-2）和恒河猴LL-37/CAP-18 （rhLL-37/ rhCAP-18）的cdna。预测的氨基酸序列与人类分子具有高度的同源性。用免疫组织化学方法分析猴抗菌肽的表达，并与3种针对人分子的多克隆抗体结合。与人类一样，恒河猴的抗微生物肽在各种器官的上皮细胞中表达，本研究表明恒河猴的β -防御素和抗菌肽与人类分子具有密切的同源性，表明非人类灵长类动物是研究先天免疫功能的有效模式生物。
One component of host defense at mucosal surfaces appears to be epithelium derived antimicrobial peptides. Molecules of the defensin and cathelicidin families have been studied in several species, including human and mouse. We describe in this report the identification and characterization of rhesus monkey homologues of human mucosal antimicrobial peptides. Using reverse transcriptase PCR methodology, we cloned the cDNAs of rhesus monkey beta -defensin 1 and 2 (rhBD-1 and rhBD-2) and rhesus monkey LL-37/CAP-18 (rhLL-37/ rhCAP-18). The predicted amino acid sequences showed a high degree of homology to the human molecules. The expression of the monkey antimicrobial peptides was analyzed using immunohistochemistry with three polyclonal antibodies to the human molecules. As in humans, rhesus monkey antimicrobial peptides are expressed in epithelia of various organs, The present study demonstrates that beta- defensins and cathelicidins of rhesus monkeys are close homologues to the human molecules and indicate that nonhuman primates represent valid model organisms to study innate immune functions.