Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR
Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR
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DOI：
10.1093/bbb/zbac123
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发表时间：
2022-07-22
影响因子：
1.6
通讯作者：
Momma, Keiko
中科院分区：
文献类型：
--
作者：
Kawai, Natsuki;Hirakawa, Yuki;Momma, Keiko
赤霉素调节蛋白（GRP）是一种水果严重过敏原。通过逆转录-定量PCR（RT-qPCR）测定针对肌动蛋白标准化的桃中GRP表达量。结果与酶联免疫吸附试验（ELISA）结果一致。GRP在果肉中的表达量明显高于果皮，且在成熟期表达量迅速增加。该方法也适用于其他植物中GRP含量的估算。
Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.