Inverse relationship of interleukin-6 and mast cells in children with inflammatory and non-inflammatory abdominal pain phenotypes.
Inverse relationship of interleukin-6 and mast cells in children with inflammatory and non-inflammatory abdominal pain phenotypes.
复制标题
DOI：
10.4291/wjgp.v3.i6.102
复制
发表时间：
2012-12-15
期刊：
影响因子：
--
通讯作者：
Youssef, Nader N
中科院分区：
文献类型：
--
作者：
Henderson, Wendy A;Shankar, Ravi;Youssef, Nader N
目的：检测腹痛患儿胃肠黏膜中白细胞介素6(IL-6)、肥大细胞、肠嗜铬细胞、5-羟色胺和P物质的表达。方法：对48例非炎症性肠病(肠易激综合征和功能性腹痛)和炎症性肠病的胃肠活检组织切片，进行IL-6、肥大细胞、肠嗜铬细胞、5-羟色胺和P物质染色。活检表型由病理学家确认，对患者信息视而不见。用描述性统计、卡方检验和独立样本t检验比较炎症性和非炎症性两组之间的差异。结果：队列中48人，平均年龄11.9岁(SD=2.9)，54.2%的女性，90%的高加索人，由非炎症性表型(n=26)和炎症性表型(n=22)组成。P物质表达与肥大细胞计数呈显著负相关(P=0.373.0 5，r=-0.0 5)。P物质在女性患者活检组织中的表达频率较高，在上胃肠道粘膜中的表达强度高于下胃肠道粘膜。炎症表型胃肠黏膜对IL-6的免疫反应性显著高于非炎症表型(P=0.004)。此外，我们还发现，与炎症性疾病组相比，非炎症性表型的粘膜肥大细胞显著增多(P=0.049)。这种差异在下结肠活检中尤其明显。结论：这项研究的发现为确定儿童未确诊腹痛的生物标志物提供了初步证据，并可能为未来的评估提供候选基因。
AIM: To investigate interleukin-6 (IL-6), mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P in the gastrointestinal mucosa of children with abdominal pain.METHODS: Formalin-fixed paraffin-embedded gastrointestinal biopsy blocks from patients (n = 48) with non-inflammatory bowel disease (irritable bowel syndrome and functional abdominal pain) and inflammatory bowel disease were sectioned and stained for IL-6, mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P. All children had chronic abdominal pain as part of their presenting symptoms. Biopsy phenotype was confirmed by a pathologist, blinded to patient information. Descriptive statistics, chi-square, and independent sample t tests were used to compare differences between the inflammatory and non-inflammatory groups.RESULTS: The cohort (n = 48), mean age 11.9 years (SD = 2.9), 54.2% females, 90% Caucasian, was comprised of a non-inflammatory (n = 26) and an inflammatory (n = 22) phenotype. There was a significant negative correlation between substance P expression and mast cell count (P = 0.05, r = -0.373). Substance P was found to be expressed more often in female patient biopsies and more intensely in the upper gastrointestinal mucosa as compared to the lower mucosa. There were significantly increased gastrointestinal mucosal immunoreactivity to IL-6 (P = 0.004) in the inflammatory phenotype compared to non-inflammatory. Additionally, we found significantly increased mast cells (P = 0.049) in the mucosa of the non-inflammatory phenotype compared to the inflammatory group. This difference was particularly noted in the lower colon biopsies.CONCLUSION: The findings of this study yield preliminary evidence in identifying biomarkers of undiagnosed abdominal pain in children and may suggest candidate genes for future evaluation.