Point mutational analysis of the Xenopus laevis 5S gene promoter.
Point mutational analysis of the Xenopus laevis 5S gene promoter.
复制标题
非洲爪蟾 5S 基因启动子的点突变分析。
作者：
T. Pieler;B. Appel;S. Oei;H. Mentzel;V. Erdmann
我们在非洲爪蟾（Xenopus laevis）体细胞5S基因编码区及其5′侧翼的GC和CG碱基对中引入了C → T转换，以分析其对转录活性和调控的影响。这些研究使我们能够区分两个启动子元件和它们的间隔区，在内部控制区。与相应tRNA共有序列不同的5′元件内的突变基本上降低转录活性，而不显著影响转录因子（TF）III A结合。间隔区的突变对转录没有明显影响。发现3′启动子元件延伸至97位，因为该区域的突变干扰转录活性。这可能至少部分归因于TF III A的竞争强度降低。
We have introduced C to T transitions into GC and CG base pairs of the Xenopus laevis somatic 5S gene coding region and its 5′ flank in order to analyse their effects on transcription activity and regulation. These studies allow us to differentiate between the two promoter elements and their spacer, within the internal control region. Mutations within the 5′ element which dissent from the corresponding tRNA consensus sequence reduce transcription activity substantially without significantly affecting transcription factor (TF) III A binding. Mutations in the spacer region have no pronounced effect on transcription. The 3′ promoter element is found to extend to position 97, since mutations in this region interfere with transcription activity. This may be, at least partially, attributable to a reduced competition strength for TF III A.