INTRACELLULAR PH AND K+ OF CARDIAC AND SKELETAL-MUSCLE IN ACIDOSIS AND ALKALOSIS
INTRACELLULAR PH AND K+ OF CARDIAC AND SKELETAL-MUSCLE IN ACIDOSIS AND ALKALOSIS
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DOI：
10.1152/ajplegacy.1975.229.5.1305
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发表时间：
1975-01-01
影响因子：
--
通讯作者：
CAMERON, IR
中科院分区：
文献类型：
--
作者：
POOLEWILSON, PA;CAMERON, IR
本文比较了代谢性酸中毒和呼吸性酸中毒对麻醉家兔心肌和骨骼肌细胞内pH（pHi）和K+的影响。分别根据[51 Cr] EDTA和[14 C]DMO的分布体积计算细胞外空间和pHi。当通过改变PCO_2改变pHe时，pHi与细胞外pH（pHe）的直线斜率大于（P <0.05--0.001）左右心室、心房、膈肌和四头肌的pHe代谢变化时的直线斜率。在代谢性酸中毒和代谢性酸中毒期间，pHi/pHe线的斜率在组织之间没有变化。在呼吸性酸中毒时，各心肌组织的斜率无差异，但左心室的斜率小于四头肌（P <0.001）。在代谢性酸中毒（P <0.05）或呼吸性酸中毒（P <0.02）时，左心室细胞内K+升高，而膈肌细胞内K+降低（P <0.02）。细胞内K+与pHe和pHE-PHi相关。pHi而非细胞内K+的变化可以解释呼吸性和代谢性酸中毒中心肌功能的已知差异。
The effects of a metabolic and respiratory acidosis and alkalosis on intracellular pH (pHi) and K+ have been compared in cardiac and skeletal muscle from the anesthetized rabbit. The extracellular space and pHi were calculated from the distribution volumes of [51Cr] EDTA and [14C]DMO, respectively. When pHe was varied by altering PCO2, the slope of the line relating pHi to the extracellular pH (pHe) was greater (P less than 0.05--0.001) than that obtained during metabolic changes of pHe in right and left ventricles, atria, diaphragm, and quadriceps. During metabolic acidosis and alkalosis, the slope of pHi/pHe line did not vary between tissues. During respiratory acidosis, there was no difference in slope between cardiac tissues, but it was less in left ventricle than quadriceps (P less than 0.001). In left ventricle intracellular K+ increased in a metabolic (P less than 0.05) or respiratory acidosis (P less than 0.02), whereas in diaphragm it decreased (P less than 0.02). Intracellular K+ correlated with pHe and pHE-PHi. Changes in pHi but not intracellular K+ could explain known differences in myocardial function in respiratory and metabolic acidosis.