Hormonal regulation of free intracellular calcium concentrations in small and large ovine luteal cells.
Hormonal regulation of free intracellular calcium concentrations in small and large ovine luteal cells.
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小和大绵羊黄体细胞中游离细胞内钙浓度的激素调节。
DOI：
10.1095/biolreprod41.4.771
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发表时间：
1989
影响因子：
3.6
通讯作者：
Niswender,GD
中科院分区：
文献类型：
--
作者：
Wiltbank,MC;Guthrie,PB;Mattson,MP;Kater,SB;Niswender,GD
介导黄体激素调节的第二信使尚未完全确定，特别是初级黄体溶解激素前列腺素F2α（PGF 2 α）。在这项研究中，通过使用计算机辅助显微镜成像的Fura-2荧光，在单个的小和大的绵羊黄体细胞中测量了卵巢诱导的细胞内游离钙浓度的变化。该技术可以很容易地检测到用1 μM钙离子载体A23187处理后小和大黄体细胞内游离钙浓度的瞬时增加。PGF 2 α（1 μM）处理导致大黄体细胞（前= 73 ± 2 nM; PGF 2 α后2 min = 370 ± 21 nM; n = 33个细胞）中游离钙浓度显著增加，但小黄体细胞（前= 66 ± 4 nM; PGF 2 α后2 min = 69 ± 8 nM; n = 12个细胞）中游离钙浓度未显著增加。钙反应的幅度和时间具有剂量和时间依赖性。PGF 2 α诱导的细胞内游离钙增加可能是由于细胞外钙内流，因为加入无机钙通道阻滞剂（100 μM锰或钴）减弱了对PGF 2 α的反应，而去除细胞外钙则消除了该反应。与PGF 2 α相反，促黄体生成素（LH）（100 ng/ml）对小或大黄体细胞内游离钙水平无影响，尽管该剂量的LH刺激小黄体细胞产生孕酮（p < 0.01）。因此，游离钙浓度的变化可能是介导PGF 2 α在大绵羊黄体细胞中的溶黄体作用的细胞内第二信息。
The second messengers mediating hormonal regulation of the corpus luteum are incompletely defined, particularly for the primary luteolytic hormone prostaglandin F2α(PGF2α). In this study, hormonally induced changes in free intracellular calcium concentrations were measured in individual small and large ovine luteal cells by using computer-assisted microscopic imaging of fura-2 fluorescence. This technique could readily detect transient increases in free calcium concentrations within both small and large luteal cells after treatment with 1 μM of the calcium ionophore, A23187. Treatment with PGF2α(1 μM) caused a dramatic increase in free calcium concentrations in large (before = 73 ± 2 nM; 2 min after PGF2α= 370 ± 21 nM; n = 33 cells) but not in small (before = 66 ± 4 nM; 2 min after PGF2α= 69 ± 8 nM; n = 12 cells) luteal cells. The magnitude and timing of the calcium response was dose- and time-dependent. The PGF2α-induced increase in free intracellular calcium is probably due to influx of extracellular calcium, since inclusion of inorganic calcium channel blockers (100 μM manganese or cobalt) attenuated the response to PGF2αand removal of extracellular calcium eliminated the response. In contrast to PGF2α, luteinizing hormone (LH) (100 ng/ml) caused no change in intracellular levels of free calcium in small or large luteal cells, even though this dose of LH stimulated (p < 0.01) progesterone production by small luteal cells. Therefore, alterations in free calcium concentrations could be the intracellular second message mediating the luteolytic action of PGF2αin the large ovine luteal cell.