Automated analysis for plasma epinephrine and norepinephrine by liquid chromatography, including a sample cleanup procedure.
Automated analysis for plasma epinephrine and norepinephrine by liquid chromatography, including a sample cleanup procedure.
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通过液相色谱法自动分析血浆肾上腺素和去甲肾上腺素，包括样品净化程序。
作者：
A. Yamatodani;H. Wada
我们描述了一个全自动的方法分析血浆肾上腺素和去甲肾上腺素通过使用一个双柱系统的“高效”液相色谱。去蛋白血浆中的儿茶酚胺在第一个（制备）色谱柱上纯化，然后自动转移到第二个（分析）色谱柱，在其中肾上腺素和去甲肾上腺素被分离。这些化合物，然后测定荧光与连续流动反应系统的三羟基吲哚法。该方法的最小灵敏度分别为0.02和0.04 pmol的肾上腺素和去甲肾上腺素。一次测定可在30分钟内完成，该程序需要超过1 mL的血浆。合并血浆色谱测定的运行内CV大于3%，两种化合物的分析总回收率为95%。医学生安静时血浆肾上腺素浓度为0.32（SE 0.06）nmol/L，去甲肾上腺素浓度为0.98（SE 0.08）nmol/L。
We describe a fully automated method of analysis for plasma epinephrine and norepinephrine by use of a two-column system of "high-performance" liquid chromatography. Catecholamines in deproteinized plasma are purified on the first (preparation) column, then transferred automatically to the second (analytical) column in which epinephrine and norepinephrine are resolved. These compounds are then determined fluorometrically with a continuous-flow reaction system by the trihydroxyindole method. The minimum sensitivity of the method is 0.02 and 0.04 pmol for epinephrine and norepinephrine, respectively. One assay can be completed in 30 min, and greater than 1 mL of plasma is required for the procedure. The within-run CV in the chromatographic determination of pooled plasma was greater than 3%, and the analytical overall recovery of the two compounds was 95%. The concentrations in plasma of medical students at rest were 0.32 (SE 0.06) nmol/L for epinephrine and 0.98 (SE 0.08) nmol/L for norepinephrine.