NUCLEOTIDE-SEQUENCE OF THE PNTA AND PNTB GENES ENCODING THE PYRIDINE-NUCLEOTIDE TRANSHYDROGENASE OF ESCHERICHIA-COLI
NUCLEOTIDE-SEQUENCE OF THE PNTA AND PNTB GENES ENCODING THE PYRIDINE-NUCLEOTIDE TRANSHYDROGENASE OF ESCHERICHIA-COLI
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DOI：
10.1111/j.1432-1033.1986.tb09802.x
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发表时间：
1986-08-01
期刊：
影响因子：
--
通讯作者：
BRAGG, PD
中科院分区：
文献类型：
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作者：
CLARKE, DM;LOO, TW;BRAGG, PD
跨越大肠杆菌吡啶核苷酸转氢酶 (pnt) 基因的 3240 个碱基对 DNA 片段已被测序。该序列包含两个开放阅读框，分别为1506和1386个碱基对的pntA和pntB，编码转氢酶α。和.beta。分别为亚单位。编码序列前面有启动子样结构，并且很可能是共转录的。每个编码序列前面都有 Shine-Dalgarno 序列。从纯化的α确定氨基末端氨基酸序列。和.beta。转氢酶的亚基。这些序列与根据 pntA 和 pntB 基因的核苷序列预测的序列一致。 53906(α)和48667(β)的预测相对分子质量接近通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳分析亚基获得的值。在每个亚基中观察到几个足够大以跨越细胞质膜的疏水区域。这些结果表明转氢酶是一种内在的膜蛋白。
A 3240-base-pair DNA fragment spanning the pyridine nucleotide transhydrogenase (pnt) genes of Escherichia coli has been sequenced. The sequence contains two open-reading frames, pntA and pntB of 1506 and 1386 base pairs, coding for the transhydrogenase .alpha. and .beta. subunits, respectively. The coding sequences are preceded by a promoter-like structure and are most likely co-transcribed. Each coding sequence is preceded by a Shine-Dalgarno sequence. The amino-terminal amino acid sequences were determined from the purified .alpha. and .beta. subunits of the transhydrogenase. These sequences agree with those predicted from the nucleoside sequences of the pntA and pntB genes. The predicted relative molecular masses of 53906 (.alpha.) and 48667 (.beta.) are close to the values obtained by analysis of the subunits by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Several hydrophobic regions large enough to span the cytoplasmic membrane were observed in each subunit. These results indicate that transhydrogenase is an intrinsic membrane protein.