A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects
复制标题
DOI：
10.3791/50440
复制
发表时间：
2013-08-01
影响因子：
1.2
通讯作者：
Liu, Xiaorong
中科院分区：
文献类型：
--
作者：
Feng, Liang;Chen, Hui;Liu, Xiaorong
青光眼是导致失明的主要原因之一，常与眼内压（IOP）升高相关。我们试图建立一种小鼠高眼压模型来模拟人类高眼压性青光眼。在此，激光照射应用于角膜利姆布斯以光凝固房水流出，诱导房角闭合。在激光治疗前后使用回弹眼压计监测眼压的变化。视动行为测试用于测量视觉能力的相应变化。显示了来自一只小鼠的代表性结果，该小鼠在激光照射后发展出持续的IOP升高。在该高眼压小鼠中观察到视力和对比敏感度降低。总之，我们的研究引入了一个有价值的模型系统，以研究神经元变性和潜在的分子机制在脑昏迷小鼠。
Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model of ocular hypertension to mimic human high-tension glaucoma. Here laser illumination is applied to the corneal limbus to photocoagulate the aqueous outflow, inducing angle closure. The changes of IOP are monitored using a rebound tonometer before and after the laser treatment. An optomotor behavioral test is used to measure corresponding changes in visual capacity. The representative result from one mouse which developed sustained IOP elevation after laser illumination is shown. A decreased visual acuity and contrast sensitivity is observed in this ocular hypertensive mouse. Together, our study introduces a valuable model system to investigate neuronal degeneration and the underlying molecular mechanisms in glaucomatous mice.