The Implication of YggT of Escherichia coli in Osmotic Regulation
The Implication of YggT of Escherichia coli in Osmotic Regulation
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DOI：
10.1271/bbb.90558
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发表时间：
2009-12-01
影响因子：
1.6
通讯作者：
Yoshimura, Tohru
中科院分区：
文献类型：
--
作者：
Ito, Tomokazu;Uozumi, Nobuyuki;Yoshimura, Tohru
缺乏Trk、Kup和Kdp三种主要K+吸收系统的大肠杆菌突变体在低K+和高Na+浓度下不能生长。据报道，一种海洋细菌，溶藻弧菌的fkuA和fkuB的引入，通过加速K+的吸收速度来弥补生长缺陷（Nakamura, Katoh, Shimizu， Matsuba和Unemoto， Biochim）。Biophys。学报，1277,201-208（1996）。我们研究了大肠杆菌未知基因的功能。yggS和yggT分别是fkuA和fkuB的同源物。大肠杆菌TK2420细胞缺乏三种K+摄取系统，在高Na+或甘露醇浓度下不能生长。生长缺陷通过单独引入yggT基因得到补偿：不需要yggS。本研究发现YggT赋予大肠杆菌细胞对渗透休克的耐受性，并讨论了其可能的机制。
An Escherichia coli mutant lacking three major K+ uptake systems, Trk, Kup, and Kdp, did not grow under low K+ and high Na+ concentrations. The introduction of fkuA and of fkuB of a marine bacterium, Vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of K+ uptake (Nakamura, Katoh, Shimizu, Matsuba, and Unemoto, Biochim. Biophys. Acta, 1277, 201-208 (1996)). We investigated the function of unknown genes of E. coli., yggS and yggT, homologs of fkuA and fkuB respectively. E. coli TK2420 cells, which lack the three K+ uptake systems, did not grow under high Na+ or mannitol concentrations. The growth defect was compensated by the introduction of the yggT gene alone: yggS was not required. Here we found that YggT endowed E. coli cells with a tolerance for osmotic shock, and discuss a possible mechanism.