Fabrication of a planar-form screen-printed solid electrolyte modified Ag/AgCl reference electrode for application in a potentiometric biosensor
Fabrication of a planar-form screen-printed solid electrolyte modified Ag/AgCl reference electrode for application in a potentiometric biosensor
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DOI：
10.1021/ac051562
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发表时间：
2006-06-15
影响因子：
7.4
通讯作者：
Chou, Tse-Chuan
中科院分区：
文献类型：
--
作者：
Liao, Wei-Yin;Chou, Tse-Chuan
本研究的特点是使用丝网印刷方法制造平面型固体电解质改性 (PSEM) Ag/AgCl 参比电极。 PSEM Ag/AgCl 参比电极使用琼脂凝胶作为内部电解质，使用氯丁橡胶作为液体接界和绝缘体。这些常见的低成本材料和简单的制造工艺使所提出的参比电极成为具有成本效益的大规模生产的理想选择。结果表明，在连续测量条件下，所开发的参比电极对大多数生理上重要的离子种类不敏感，包括Na+、K+、Li+、Ca2+、NH4+和Cl-。此外，与传统的商业参比电极一样，所提出的参比电极表现出可逆响应，这种响应一直保持到琼脂凝胶干燥为止。 PSEM Ag/AgCl参比电极与氧化铱修饰的Pt基pH指示电极集成，形成芯片式pH生物传感器。该生物传感器的性能与基于宏观商用 Ag/AgCl 参比电极的 pH 计获得的性能一致。实验结果证实，所提出的生物传感器能够提供各种实际样品的精确 pH 测量。因此，本研究中提出的 PSEM Ag/AgCl 参比电极为许多传统基于芯片的 pH 传感器中使用的宏观 Ag/AgCl 参比电极提供了可行的替代方案。
This study features the fabrication of a planar-form, solid electrolyte modified, (PSEM) Ag/AgCl reference electrode using a screen-printing method. The PSEM Ag/AgCl reference electrode uses agar gel as the inner electrolyte and chloroprene rubber for the liquid junction and insulator. These common low-cost materials and the simple fabrication processes involved render the proposed reference electrode an ideal candidate for cost-efficient mass production. It is shown that the developed reference electrode is insensitive to most of the physiologically important ionic species, including Na+, K+, Li+, Ca2+, NH4+, and Cl-, under continuous measurement conditions. Moreover, as with conventional commercial reference electrodes, the proposed reference electrode exhibits a reversible response, which is maintained until the agar gel dries out. The PSEM Ag/AgCl reference electrode is integrated with an iridium oxide modified Pt-based pH indicator electrode to form a chip-type pH biosensor. The performance of this biosensor is consistent with that obtained from a pH meter based on a macroscopic commercial Ag/AgCl reference electrode. The experimental results confirm that the proposed biosensor is capable of providing precise pH measurements of various real samples. Accordingly, the PSEM Ag/AgCl reference electrode presented in this study provides a viable alternative to the macroscopic Ag/AgCl reference electrode used in many conventional chip-based pH sensors.