Crystallization and preliminary X-ray analysis of L-serine 3-dehydrogenase complexed with NADP+ from the hyperthermophilic archaeon Pyrobaculum calidifontis,
Crystallization and preliminary X-ray analysis of L-serine 3-dehydrogenase complexed with NADP+ from the hyperthermophilic archaeon Pyrobaculum calidifontis,
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来自超嗜热古菌 Pyrobaculum calidifontis 的 L-丝氨酸 3-脱氢酶与 NADP 复合物的结晶和初步 X 射线分析，
DOI：
10.1107/s1744309112051391
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Yoneda;K.;Sakuraba;H.;Araki;T.;Shibata;T.;Nikki;T. & Ohshima;T.
以硫酸铵为沉淀剂，采用坐滴气相扩散法从超嗜热古菌Pyrobaculum calidifontis中分离出一种NAD（P）+依赖的L-丝氨酸3-脱氢酶。晶体属单斜晶系，空间群C2，晶胞参数a = 120.81，B = 57.40，c = 56.37 °，β = 106.88°。 在Photon Factory的光束线NE 3A上收集衍射数据，分辨率为1.57μ m。总体Rmerge为4.2%，数据完整性为90.1%。
An NAD(P)+-dependent l-serine 3-dehydrogenase from the hyperthermophilic archaeon Pyrobaculum calidifontis was crystallized using the sitting-drop vapour-diffusion method with ammonium sulfate as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 120.81, b = 57.40, c = 56.37 Å, β = 106.88°. Diffraction data were collected to 1.57 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 4.2% and the data completeness was 90.1%.