Thioproline Serves as an Efficient Antioxidant Protecting Human Cells from Oxidative Stress and Improves Cell Viability
Thioproline Serves as an Efficient Antioxidant Protecting Human Cells from Oxidative Stress and Improves Cell Viability
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DOI：
10.1021/acs.chemrestox.0c00055
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发表时间：
2020-07-20
影响因子：
4.1
通讯作者：
Chan, Wan
中科院分区：
文献类型：
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作者：
Ham, Yat-Hing;Chan, K. K. Jason;Chan, Wan
氧化应激与许多退行性人类疾病的病理生理学相关，包括阿尔茨海默病、动脉粥样硬化、帕金森病和癌症。我们在之前的研究中发现，暴露于氧化剂的细胞中会产生硫代脯氨酸（SPro），一种脯氨酸类似物。与先前的观察，SPro作为一个有效的腈捕获剂，我们在这项研究中测试的假设，这种氧化应激产生的半胱氨酸-甲醛加合物，SPro，可以作为一种抗氧化剂保护细胞免受氧化应激。有趣的是，结果表明，HeLa细胞培养在SPro补充培养基更耐受氧化应激，细胞活力的剂量依赖性增加。对所观察到的细胞对氧化应激耐受性增加的分子机制的调查显示SPro通过牺牲氧化作为有效的抗氧化剂。结果还表明，SPro已被纳入细胞蛋白质和诱导的蛋白质表达谱的变化处理的细胞。尽管尚未确定个体因素对观察到的细胞对氧化应激的耐受性增加的参与，但这项研究揭示了SPro作为膳食补充剂用于保护人类免受氧化应激相关的退行性人类疾病的潜在用途。
Oxidative stress is associated with the pathophysiology of many degenerative human diseases, including Alzheimer's disease, atherosclerosis, Parkinson's disease, and cancers. We discovered in our previous study that thioproline (SPro), a proline analogue, is generated in oxidant-exposed cells. With the prior observation that SPro served as an efficient nitrile trapping agent, we tested in this study the hypothesis that this oxidative stress generated cysteine-formaldehyde adduct, SPro, may serve as an antioxidant protecting cells from oxidative stress. Interestingly, results showed that HeLa cells cultured in SPro-supplemented culture media are more tolerant of oxidative stress, indicated by a dosage-dependent increase in cell viability. Investigation of the molecular mechanism of the observed increase in cell tolerance to oxidative stress revealed SPro acting as an effective antioxidant by sacrificial oxidation. Results also showed that SPro had been incorporated into cellular proteins and induced changes in protein expression profiles of treated cells. Despite being yet to determine the participation of individual factors to the observed increase of cell tolerance to oxidative stress, this study sheds light on the potential use of SPro as a dietary supplement for protecting humans from oxidative stress-associated degenerative human diseases.