Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.
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DOI：
10.1186/s13567-015-0246-4
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发表时间：
2015-09-25
影响因子：
4.4
通讯作者：
Glass EJ
中科院分区：
文献类型：
--
作者：
Corripio-Miyar Y;Hope J;McInnes CJ;Wattegedera SR;Jensen K;Pang Y;Entrican G;Glass EJ
基于表面分子的差异表达，几种哺乳动物物种中的循环单核细胞可以细分为功能不同的亚群。我们证实，牛单核细胞表达CD 172 a和MHC II类，具有两种不同的CD 14 + CD 16低/-CD 163+和CD 14 − CD 16 ++ CD 163低-细胞群体，以及更弥散的CD 14 + CD 16 + CD 163+细胞群体。相比之下，绵羊单核细胞仅由主要的CD 14 + CD 16+亚群和非常低百分比的CD 14 − CD 16 ++细胞组成。牛亚群表达相似水平的CD 80、CD 40和CD 11 c分子以及编码CD 115的mRNA。然而，进一步的mRNA分析显示，CD 14 − CD 16 ++单核细胞是CX 3CR 1highCCR 2low，而主要的CD 14+亚群是CX 3CR 1 lowCCR 2 high。前者为CD 1b阳性，CD 11b和CD 86水平低于CD 14+单核细胞。更弥散的CD 14 + CD 16+群体通常表达这些分子的中间水平。所有三个群体均通过产生白细胞介素（IL）-1β对苯酚提取的脂多糖（LPS）刺激作出反应，其中CD 16 ++亚群表达较高水平的IL-12和较低水平的IL-10。与其他单核细胞群体相比，CD 14 − CD 16 ++细胞更具内吞性，并诱导更大的同种异体T细胞应答。总之，数据显示了单核细胞的经典、中间和非经典定义之间的相似性和差异，如对其他哺乳动物种属所述，以及其他潜在亚群。对这些单核细胞群的进一步功能分析可能有助于解释反刍家畜中感染、炎症和疫苗接种的动物间和种间差异。
Circulating monocytes in several mammalian species can be subdivided into functionally distinct subpopulations based on differential expression of surface molecules. We confirm that bovine monocytes express CD172a and MHC class II with two distinct populations of CD14+CD16low/-CD163+ and CD14−CD16++CD163low- cells, and a more diffuse population of CD14+CD16+CD163+ cells. In contrast, ovine monocytes consisted of only a major CD14+CD16+ subset and a very low percentage of CD14−CD16++cells. The bovine subsets expressed similar levels of CD80, CD40 and CD11c molecules and mRNA encoding CD115. However, further mRNA analyses revealed that the CD14−CD16++ monocytes were CX3CR1highCCR2low whereas the major CD14+ subset was CX3CR1lowCCR2high. The former were positive for CD1b and had lower levels of CD11b and CD86 than the CD14+ monocytes. The more diffuse CD14+CD16+ population generally expressed intermediate levels of these molecules. All three populations responded to stimulation with phenol-extracted lipopolysaccharide (LPS) by producing interleukin (IL)-1β, with the CD16++ subset expressing higher levels of IL-12 and lower levels of IL-10. The CD14−CD16++ cells were more endocytic and induced greater allogeneic T cell responses compared to the other monocyte populations. Taken together the data show both similarities and differences between the classical, intermediate and non-classical definitions of monocytes as described for other mammalian species, with additional potential subpopulations. Further functional analyses of these monocyte populations may help explain inter-animal and inter-species variations to infection, inflammation and vaccination in ruminant livestock.