IL-1 and IL-4 as reciprocal regulators of IL-2 induced lymphocyte cytotoxicity.
IL-1 and IL-4 as reciprocal regulators of IL-2 induced lymphocyte cytotoxicity.
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IL-1 和 IL-4 作为 IL-2 诱导的淋巴细胞细胞毒性的相互调节剂。
DOI：
10.1038/bjc.1990.341
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发表时间：
1990
影响因子：
8.8
通讯作者：
Golub,SH
中科院分区：
文献类型：
--
作者：
Ebina,N;Gallardo,D;Shau,H;Golub,SH
白细胞介素4 （IL-4）抑制白细胞介素2 （IL-2）诱导的人外周血单核细胞（PBMC）淋巴因子活化杀伤细胞（LAK）的发育。IL-2在高（1,000 U ml-1）和低（10 U ml-1）浓度下均有抑制作用。IL-4需要在IL-2培养开始时就存在，才能发挥抑制作用。IL-4还能抑制IL-2培养的PBMC上CD25 （Tac）抗原的发展。在IL-2培养早期加入白细胞介素1 （IL-1）可逆转IL-4对LAK诱导的抑制作用。IL-1增强IL-2诱导的LAK发育，这可能部分解释了IL-1对IL-4抑制的逆转。IL-1也逆转了IL-4对CD25抗原表达的抑制作用，尽管IL-1单独不能增强IL-2培养的PBMC对CD25表达的诱导。此外，IL-4抑制IL-2诱导的PBMC中IL-1的产生。因此，抑制CD25可能是抑制LAK诱导的一个途径。CD56的表达与LAK活性的表达无直接关系。IL-4、IL-1或两者联合使用对IL-2诱导的CD56表达无影响。这些结果表明，IL-4在il -2诱导的LAK发育中具有拮抗作用，IL-1在il -2诱导的LAK发育中具有协同作用。
Interleukin 4 (IL-4) suppresses the interleukin 2 (IL-2) induced lymphokine-activated killer (LAK) cell development from human peripheral blood mononuclear cells (PBMC). Suppression is observed at high (1,000 U ml-1) as well as low (10 U ml-1) concentrations of IL-2. IL-4 needs to be present at the beginning of the IL-2 culture to exert the suppressive effect. IL-4 also inhibits the development of CD25 (Tac) antigen on the PBMC cultured in IL-2. Interleukin 1 (IL-1) can reverse the suppressive effect of IL-4 on LAK induction when added at the early phase of the IL-2 culture. IL-1 enhances IL-2 induced LAK development, which may partially explain the reversion of IL-4 inhibition by IL-1. IL-1 also reverses the inhibitory effect of IL-4 on the development of CD25 antigen expression, although IL-1 alone does not enhance the induction of CD25 expression in PBMC cultured by IL-2. Furthermore, IL-4 suppresses IL-2 induced IL-1 production in PBMC. Thus, suppression of CD25 may be a pathway for the suppression of LAK induction. The expression of CD56 is not directly associated with the expression of LAK activity. IL-4, IL-1 or combination of the two cytokines has no effect on IL-2 induced expression of CD56. These results indicate that IL-4 has an antagonistic effect and IL-1 has a synergistic effect on IL-2-induced LAK development.