Influence of oxidative stress on the antibacterial activity of betulin, betulinic acid and ursolic acid
Influence of oxidative stress on the antibacterial activity of betulin, betulinic acid and ursolic acid
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DOI：
10.1016/j.micpath.2017.08.012
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发表时间：
2017-10-01
影响因子：
3.8
通讯作者：
Ajiboye, T. O.
中科院分区：
文献类型：
--
作者：
Oloyede, H. O. B.;Ajiboye, H. O.;Ajiboye, T. O.
研究了活性氧自由基和氧化应激在桦木醇、桦木酸和熊果酸对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抗菌活性中的作用。测定了白桦脂醇、白桦脂酸和熊果酸对大肠杆菌的最低抑菌浓度。coli、铜绿假单胞菌和S.金黄色葡萄球菌分别为1024、256和1024 μ g/mL; 512、256和256 μ g/mL; 256、256和64 μ g/mL。桦木醇，桦木酸和熊果酸处理的细菌的细胞活力下降的时间依赖性的方式。在2，2 '-联吡咯烷基存在下处理细菌增加了细胞活力。白桦脂醇、白桦脂酸和熊果酸处理的细菌细胞产生超氧阴离子自由基显著增加（p < 0.05）。白桦脂醇、白桦脂酸和熊果酸处理的细菌中NAD（+）/NADH比值显著增加（p < 0.05）。同样，E. coli、铜绿假单胞菌和S.白桦脂醇、白桦脂酸和熊果酸处理后，金黄色葡萄球菌的谷胱甘肽二硫化物、丙二醛和DNA片段含量显著降低，相应增加。以上结果表明，白桦脂醇、白桦脂酸和熊果酸增强了E. coli、铜绿假单胞菌和S.金黄色葡萄球菌导致增加的ROS产生、芬顿反应、脂质过氧化、DNA片段化和相应的细菌死亡。(C)2017爱思唯尔有限公司版权所有
Contribution of reactive oxygen species and oxidative stress in the antibacterial activities of betulin, betulinic acid and ursolic acid against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus was investigated. The minimum inhibitory concentrations of betulin, betulinic acid and ursolic acid against E. coli, P. aeruginosa and S. aureus are 1024-, 256- and 1024-mu g/mL; 512-, 256- and 256 mu g/mL; 256-, 256- and 64 mu g/mL respectively. Cell viability of betulin-, betulinic acid- and ursolic acid-treated bacteria decrease in time dependent manner. Treatment of bacteria in the presence of 2,2'-bipyrydyl increased cell viability. Superoxide anion radical production increased significantly (p < 0.05) in bacterial cells-treated with betulin, betulinic acid and ursolic acid. Furthermore, NAD(+)/NADH ratio increased significantly (p < 0.05) in betulin-, betulinic acid- and ursolic acid-treated bacteria. Similarly, level of reduced glutathione in E. coli, P. aeruginosa and S. aureus decreased significantly with corresponding increase in glutathione disulphide, malondialdehyde and fragmented DNA following betulin, betulinic acid and ursolic acid treatments. It is evident from the above findings that betulin, betulinic acid and ursolic acid enhanced electron transport chain activity in E. coli, P. aeruginosa and S. aureus leading to increased ROS generation, Fenton reaction, lipid peroxidation, fragmented DNA and consequentially bacterial death. (C) 2017 Elsevier Ltd. All rights reserved.