Expression analysis of BACE2 in brain and peripheral tissues
Expression analysis of BACE2 in brain and peripheral tissues
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DOI：
10.1074/jbc.m002688200
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发表时间：
2000-07-07
影响因子：
4.8
通讯作者：
Vassar, R
中科院分区：
文献类型：
--
作者：
Bennett, BD;Babu-Khan, S;Vassar, R
β - 位点淀粉样前体蛋白裂解酶（BACE）是一种新型跨膜天冬氨酸蛋白酶，具有参与阿尔茨海默病的β - 分泌酶的所有已知特征（瓦萨尔，R.；贝内特，B. D.；巴布 - 汗，S.；卡恩，S.；门迪亚兹，E. A.；丹尼斯，P.；特普洛，D. B.；博斯，S.；阿马兰特，P.；勒洛夫，R.；罗，Y.；费舍尔，S.；富勒，J.；埃德森，S.；莱尔，J.；亚罗辛斯基，M. A.；比尔，A. L.；柯伦，E.；伯吉斯，T.；路易斯，J. - C.；柯林斯，F.；特里诺，J.；罗杰斯，G.；西特龙，M.（1999年）《科学》286，735 - 741页）。我们分析了一种被称为BACE2的BACE同源物的序列和表达模式。BACE和BACE2在天冬氨酸蛋白酶中是独特的，因为它们具有一个带有预测跨膜区域的羧基末端延伸部分，并且它们共同定义了一个新的家族。Northern分析显示，BACE2 mRNA在大多数人体外周组织中低水平表达，在结肠、肾脏、胰腺、胎盘、前列腺、胃和气管中表达水平较高。成人和胎儿全脑以及大多数成人大脑亚区表达的BACE2 mRNA水平非常低或检测不到。此外，成年大鼠脑的原位杂交显示，BACE2 mRNA在大多数脑区的表达水平非常低。大脑中BACE2 mRNA极低或检测不到的水平与预测的β - 分泌酶的表达模式不一致。
Beta-site amyloid precursor protein cleaving enzyme (BACE) is a novel transmembrane aspartic protease that possesses all the known characteristics of the beta-secretase involved in Alzheimer's disease (Vassar, R., Bennett, B. D., Babu-Khan, S., Kahn, S., Mendiaz, E. A., Denis, P., Teplow, D. B., Boss, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M. A., Biere, A. L., Curran, E., Burgess, T., Louis, J.-C., Collins, F., Treanor, J., Rogers, G., and Citron, M. (1999) Science 286, 735-741). We have analyzed the sequence and expression pattern of a BACE homolog termed BACE2. BACE and BACE2 are unique among aspartic proteases in that they possess a carboxyl-terminal extension with a predicted transmembrane region and together they define a new family. Northern analysis reveals that BACE2 mRNA is expressed at low levels in most human peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach, and trachea. Human adult and fetal whole brain and most adult brain subregions express very low or undetectable levels of BACE2 mRNA In addition, in situ hybridization of adult rat brain shows that BACE2 mRNA is expressed at very low levels in most brain regions. The very low or undetectable levels of BACE2 mRNA in the brain are not consistent with the expression pattern predicted for beta-secretase.